Maize Inbred NPNA0003 Marker-Assisted Breeding

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Solution Overview

Problem

Current corn breeding methods face challenges in producing high-yielding, agronomically sound plants that are uniformly adapted across the U.S. Corn Belt, as regional growing conditions and specific issues like Gray Leaf Spot infection, cool temperatures, and soil pH levels require region-specific traits, and existing methods struggle to introduce desired traits efficiently across broad regions.

Innovation Solution

The development of the maize inbred plant NPNA0003, which includes a mutant or transgenic gene conferring traits such as herbicide resistance, insect resistance, and disease resistance, and a process for producing F1 hybrid maize seeds by crossing NPNA0003 with other maize plants, allowing for the introduction and stabilization of desired traits through backcrossing and selection, enabling the creation of hybrids with improved adaptability and performance across diverse conditions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop maize varieties, then regional adaptation may be achieved, but the ability to uniformly introduce desired traits across broad regions is limited

Engineering Contradiction:
Improveregional adaptationVSAvoidtrait introduction efficiency
Core Design Contradiction:
Adaptability or versatilityVSProductivity

Solution Approach 1:

The patent utilizes molecular markers to detect and track specific genetic parameters (desired traits) during breeding, enabling precise selection and uniform introduction of traits across diverse regions while maintaining regional adaptability through marker-assisted selection programs

Inventive Principle:
Principle #35Parameter changes

2Stability of the object's composition

If inbred lines are used as parents for hybrid production, then uniformity and reproducibility of hybrid seed are achieved, but the inbred plants themselves show decreased vigor

Engineering Contradiction:
Improvehybrid seed uniformityVSAvoidinbred plant vigor
Core Design Contradiction:
Stability of the object's compositionVSReliability

Solution Approach 1:

The patent employs molecular markers to copy and track desired genetic traits through multiple generations of breeding, allowing selection of inbred lines that maintain both the uniformity needed for hybrid production and the vigor required for practical use, by verifying genetic composition at the molecular level

Inventive Principle:
Principle #26Copying

3Adaptability or versatility

If multiple breeding stages and selections are performed to develop novel plant germplasm, then desirable traits are combined, but the breeding process becomes complex and time-consuming

Engineering Contradiction:
Improvecombination of desirable traitsVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent implements marker-assisted selection that provides continuous feedback on the genetic composition of breeding populations, allowing breeders to track the accumulation of desirable traits through multiple generations and make informed selection decisions, thereby streamlining the complex breeding process while ensuring comprehensive trait combination

Inventive Principle:
Principle #23Feedback

Data Source

PatentUS8735691B2Variety corn line NPNA0003
Publication Date: 2014.05.27 SYNGENTA CROP PROTECITON AG

AI summary

The present invention provides an inbred corn line designated NPNA0003, methods for producing a corn plant by crossing plants of the inbred line NPNA0003 with plants of another corn plant. The invention further encompasses all parts of inbred corn line NPNA0003, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line NPNA0003, and plants produced according to these methods.