Canola Line NS6151 Cytoplasmic Male Sterility Breeding

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Solution Overview

Problem

Current canola breeding techniques face challenges in predicting and achieving desirable traits such as disease resistance, drought tolerance, and uniformity, due to the unpredictability of genetic combinations and the need for extensive research and resources to develop superior varieties.

Innovation Solution

The development of a novel Brassica napus line, NS6151, which incorporates cytoplasmic male sterility and can be used for crossing with other Brassica plants, allowing for the introgression of desired traits through backcross conversion and transformation, enabling the creation of hybrid canola seeds with improved agronomic characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional canola breeding techniques are used, then existing varieties can be maintained, but desirable traits such as disease resistance, drought tolerance, and uniformity cannot be reliably achieved due to unpredictability of genetic combinations

Engineering Contradiction:
Improvepredictability of desirable traitsVSAvoidcomplexity of breeding process
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent introduces a cytoplasmic male sterility (CMS) system as an intermediary mechanism to control pollination and enable directed breeding. The CMS line acts as a female parent that cannot self-pollinate, forcing cross-pollination with selected male parents, thereby providing reliable control over genetic combinations and enabling predictable inheritance of desirable traits.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The breeding program is segmented into distinct components: a CMS female line (NS6151) and selected male lines. This segmentation allows independent selection and optimization of each parent line's genetics, with the CMS line providing maternal effects and pollination control, while male lines contribute specific desirable traits through controlled crosses.

Inventive Principle:
Principle #1Segmentation

2Productivity

If extensive research and resources are invested in traditional breeding, then superior varieties can be developed, but the process is time-consuming and resource-intensive

Engineering Contradiction:
Improverate of variety developmentVSAvoidtime required for breeding
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The patent employs preliminary action by establishing a CMS line (NS6151) with desirable maternal traits and pollination control capability before initiating the breeding program. This pre-established CMS line serves as a ready-to-use female parent that can immediately enable controlled crosses with selected male lines, reducing the time required compared to developing both parents from scratch.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The CMS line provides self-service by automatically ensuring cross-pollination through its male sterility characteristic. The system self-regulates pollination by preventing self-fertilization and requiring external pollen application from selected male parents, thereby eliminating the need for complex pollination management and reducing breeding program complexity.

Inventive Principle:
Principle #25Self-service

3Stability of the object's composition

If conventional breeding methods are used, then existing canola varieties can be maintained, but uniformity of plant characteristics such as germination, stand establishment, growth rate, and maturity cannot be reliably achieved

Engineering Contradiction:
Improveuniformity of plant characteristicsVSAvoidease of achieving uniformity
Core Design Contradiction:
Stability of the object's compositionVSEase of operation

Solution Approach 1:

The breeding program incorporates feedback mechanisms where the uniformity and performance of progeny are evaluated and used to select subsequent parents. The CMS line's controlled pollination allows for systematic evaluation of inheritance patterns, enabling selection of male lines that produce uniform progeny with desired characteristics such as consistent germination, stand establishment, growth rate, and maturity.

Inventive Principle:
Principle #23Feedback

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach allows for the production of canola hybrids with enhanced traits like disease resistance, improved yield, and uniformity, reducing the complexity and cost associated with traditional breeding methods by ensuring genetic stability and predictability.

Implementation Method 1

This invention includes NS6151 with cytoplasm comprising a gene or genes that cause male sterility

Methodology Applied
Scientific EffectCytoplasmic male sterility:

Data Source

PatentUS8865973B1Canola line NS6151
Publication Date: 2014.10.21 PIONEER HI BREED INTERNATIONAL INC

AI summary

A novel canola variety designated NS6151 and seed, plants and plant parts thereof. Methods for producing a canola plant that comprise crossing canola variety NS6151 with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into NS6151 through backcross conversion and/or transformation, and to the canola seed, plant and plant part produced thereby. Hybrid canola seed, plant or plant part produced by crossing the canola variety NS6151 or a locus conversion of NS6151 with another canola variety.