Nucleic Acid Amplification Disk with Temperature-Sensitive Polymer
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Solution Overview
Problem
Traditional nucleic acid amplification analyzers are expensive, require skilled operators, and struggle with accurate temperature measurement within rotating disk chambers, limiting their accessibility and accuracy in nucleic acid amplification processes.
Innovation Solution
Integration of temperature-sensitive polymer synthesis on a compact disk apparatus for real-time temperature measurement and control within the nucleic acid amplification disk, enabling direct DNA sample temperature measurement and automated nucleic acid amplification processes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If traditional nucleic acid amplification analyzers are used, then amplification function is achieved, but cost and operational complexity increase
Solution Approach 1:
The patent combines multiple nucleic acid amplification processes (cell lysis, DNA extraction, reverse transcription, amplification, and detection) into a single integrated disk apparatus. This merging eliminates the need for separate equipment and skilled operators for each step, reducing both cost and operational complexity while maintaining amplification functionality.
Solution Approach 2:
The disk apparatus is designed as a universal platform that performs multiple functions: mechanical disruption of cells, separation of nucleic acids, enzymatic reactions, and optical detection. This multi-functionality allows a single device to replace traditional multi-step workflows requiring specialized equipment and expertise.
2Measurement precision
If indirect temperature measurement is used in rotating disk chambers, then temperature control is achieved, but measurement accuracy deteriorates
Solution Approach 1:
The patent introduces a temperature-sensitive polymer as an intermediary substance within the reaction chamber. This polymer's physical properties change predictably with temperature, allowing direct optical measurement of the actual sample temperature during rotation. This intermediary enables accurate temperature monitoring without complex sensor integration in the rotating chamber.
Solution Approach 2:
The patent replaces mechanical temperature sensors (which would be difficult to implement in rotating chambers) with an optical measurement system. The temperature-sensitive polymer converts thermal information into optical signals that can be detected externally, substituting a mechanical sensing approach with an optical one that is compatible with the rotating disk design.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution provides an affordable, automated, and accurate method for nucleic acid amplification, enhancing the efficiency and precision of DNA or RNA amplification from various samples, such as blood, viruses, and bacterial materials, facilitating broader diagnostic applications.
Implementation Method 1
by integrating the temperature sensitive polymer synthesis in the disk chamber and controlling the temperature in the chamber by feed backing the temperature into the temperature controller
Data Source
AI summary
A nucleic acid amplification disk apparatus using a temperature sensitive polymer synthesis and the analysis method using the same, and more specifically, and the nucleic acid amplification device, and the analysis method using the nucleic acid amplification disk unit and the nucleic acid amplification disk for amplifying the Bacterial DNA or RNA, and the driving control section for controlling the nucleic acid amplification disk.