Nucleic Acid Analysis Device Artifact Correction
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Solution Overview
Problem
Existing nucleic acid analysis methods face challenges in accurately detecting target DNA due to artifacts such as shading and optical crosstalk, which complicate processing steps and reduce detection accuracy.
Innovation Solution
A nucleic acid analysis device and method that includes a distribution unit, labeling unit, information acquisition unit, and information processing unit to distribute samples into separated compartments, differentiate between target and non-target nucleic acid compartments, acquire and correct fluorescence information, and identify compartments with significant changes, thereby reducing artifacts and improving detection accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If optical crosstalk correction is performed by calculating artifact components from every well, then measurement precision is improved, but device complexity increases
Solution Approach 1:
The patent segments the image into multiple well regions and processes each well independently to calculate artifact components. By dividing the overall correction task into smaller per-well segments, the system achieves comprehensive optical crosstalk correction without requiring complex global processing of all wells simultaneously, thus improving measurement precision while managing device complexity.
Solution Approach 2:
The patent performs preliminary acquisition of artifact components from surrounding wells before finalizing the correction for each well. By pre-calculating the artifact components from adjacent wells and storing them for later use in correction, the system streamlines the processing workflow and reduces the computational complexity of the final correction step while maintaining high measurement precision.
2Measurement precision
If artifact correction processing is performed, then measurement precision is improved, but loss of time increases
Solution Approach 1:
The patent calculates and stores artifact components from surrounding wells in advance before the final correction step. This preliminary calculation allows the actual correction process to use pre-computed values, significantly reducing the time required for the correction operation while maintaining high measurement precision through comprehensive artifact removal.
Solution Approach 2:
The patent creates a corrected image by copying and combining the original image with calculated artifact component images. This copying approach allows the system to preserve the original data while generating a corrected version, enabling efficient processing without requiring complex in-place modifications, thus reducing processing time while maintaining accuracy.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution effectively reduces artifacts in fluorescence detection, enhancing the accuracy of nucleic acid analysis by correcting for shading and optical crosstalk, allowing for precise identification of target nucleic acid without amplification and shortening signal saturation time.
Implementation Method 1
when a reporter molecule in which a fluorescent substance and a quencher are linked to each other by single-stranded DNA is added to a reaction system, Cas12a cleaves the single-stranded DNA of the reporter molecule by a trans-cleavage reaction. Thus, the fluorescent substance and the quencher are separated, and fluorescence is generated.
Data Source
AI summary
Provided is a nucleic acid analysis device with which artifact components are reduced. The nucleic acid analysis device includes a distribution unit, a labeling unit, an information acquisition unit, and an information processing unit. The information processing unit includes an information extraction unit, an artifact component determination unit, a correction unit, and an identifying unit. The artifact component determination unit determines an artifact component from extracted information corresponding to a portion other than a plurality of individual separated compartments. The correction unit uses the artifact component to correct extracted information corresponding to the plurality of individual separated compartments.