Nucleic Acid Barcodes for Proteomic Variant Identification
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Solution Overview
Problem
Current methods for analyzing complex pools of genetic variants lack the ability to distinguish individual variants effectively, making it difficult to connect specific encoded molecular phenotypes with their corresponding genotypes.
Innovation Solution
The use of origin-specific nucleic acid barcodes allows for the high-throughput labeling and identification of target molecules, enabling the determination of their identity, quantity, and activity by sequencing, and associating genotype characteristics with phenotype characteristics while maintaining information about sample origin.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If high complexity pools of genetic variants are constructed, then the potential for exploration of design space is enormous, but the ability to distinguish individual variants and connect phenotypes with genotypes becomes difficult
Solution Approach 1:
The patent segments the complex pool of genetic variants into individually identifiable units by assigning unique nucleic acid barcodes to each variant. This segmentation allows high-throughput sequencing to distinguish and track individual variants throughout complex pools, resolving the contradiction between exploring diverse design spaces and maintaining the ability to detect specific variants.
Solution Approach 2:
The patent introduces nucleic acid barcodes as intermediary molecules that mediate between genotype and phenotype detection. These barcodes serve as detectable proxies that can be sequenced to identify specific variants, enabling the connection between genetic design and molecular phenotype without directly observing the complex genetic structures.
2Reliability
If traditional methods are used to analyze complex genetic pools, then the analysis can be performed, but the process is expensive and laborious
Solution Approach 1:
The patent replaces traditional mechanical and manual analysis methods with automated high-throughput nucleic acid sequencing. By substituting labor-intensive procedures with automated sequencing technology, the system maintains reliable analysis capability while dramatically improving productivity and reducing costs.
Solution Approach 2:
The patent changes the detection parameter from direct observation of complex genetic structures to sequencing of simple nucleic acid barcodes. This parameter change enables high-throughput automated analysis, transforming the process from expensive and laborious to efficient and scalable.
3Productivity
If individual variants are tracked through high-throughput sequencing, then multiplex analysis of diverse genotypes and phenotypes is enabled, but the complexity of the system increases
Solution Approach 1:
The patent extracts the identification function from the complex genetic pool and concentrates it into simple nucleic acid barcodes. By taking out the essential identification capability and embedding it in these simple molecular tags, the system achieves high-throughput multiplex analysis while keeping the actual analysis process relatively simple despite the complexity of the biological system.
Data Source
AI summary
The disclosure provides methods and compositions useful for labeling of target molecules with origin-specific nucleic acid identifiers (for example, barcodes), which can be used subsequently to identify, quantify, or otherwise characterize a feature or activity of target molecules originating from a particular discreet volume. Such target molecules can include polypeptides expressed by cells, in which nucleic acid molecules encoding the polypeptides are labeled with the same, or matched, origin-specific nucleic acid identifiers.