Nucleic Acid Condensing Peptide for Efficient Delivery
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Solution Overview
Problem
Current methods for delivering nucleic acids into cells, such as using cationic liposomes, face challenges in efficiently encapsulating nucleic acids due to their anionic nature, leading to suboptimal delivery efficiency and stability.
Innovation Solution
A nucleic acid condensing peptide with specific sequences, including RRRRRR and RQRQR terminals, is used to condense nucleic acids, allowing for efficient encapsulation and subsequent release within cells, enhancing delivery efficiency by forming stable complexes with cationic properties.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If cationic liposome is used to deliver nucleic acid, then the cell membrane accumulation is improved, but the nucleic acid encapsulation efficiency deteriorates due to strong electrostatic attraction between anionic nucleic acid and cationic liposome surface
Solution Approach 1:
The patent introduces a peptide as an intermediary substance that mediates between the cationic liposome and anionic nucleic acid. The peptide binds to both components, forming a bridge that enables nucleic acid encapsulation without direct electrostatic attraction between the liposome and nucleic acid, thus resolving the contradiction between membrane accumulation and encapsulation efficiency
Solution Approach 2:
The invention creates a composite system comprising cationic liposome, peptide, and nucleic acid. This composite structure allows the nucleic acid to be encapsulated within the liposome through the peptide mediator, achieving both effective cell membrane accumulation and high encapsulation efficiency simultaneously
2Quantity of substance
If peptide binds nucleic acid to enable liposome encapsulation, then the nucleic acid encapsulation efficiency is improved, but the delivery efficiency to cell deteriorates due to reduced cell membrane interaction
Solution Approach 1:
The patent applies local quality by designing the peptide with distinct functional regions: one region binds to the cationic liposome while another region interacts with the anionic nucleic acid. This localized functional differentiation allows the peptide to simultaneously facilitate both encapsulation and cell membrane interaction, resolving the contradiction between encapsulation efficiency and delivery efficiency
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The peptide effectively condenses and delivers nucleic acids into cells, improving transfection efficiency and stability of the nucleic acid delivery carriers, allowing for higher expression of the nucleic acids within the cells.
Implementation Method 1
a nucleic acid condensing peptide which condenses nucleic acid... The peptide comprises a terminal having a first sequence RRRRRR and another terminal having a second sequence RQRQR
Implementation Method 2
delivering the nucleic acid to the cell by endocytosis or the like
Data Source
AI summary
According to one embodiment, a nucleic acid condensing peptide includes one terminal having a first sequence RRRRRR and another terminal having a second sequence RQRQR. Between the first sequence and the second sequence, none or one or more intermediate sequences each consisting of RRRRRR or RQRQR are included. Further, of the first sequence, the second sequence and the intermediate sequences, two or more neutral amino acids are included between any two sequences adjacent to each other.