Nucleic Acid Extraction Tablet Using Magnesium Silicate Adsorption
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Solution Overview
Problem
Current methods for isolating and purifying nucleic acids from food samples are laborious, time-consuming, and require expensive and hazardous chemicals, making them inefficient for diverse sample compositions and processed food matrices.
Innovation Solution
A composition and method using a mixture of water-insoluble hydrated magnesium silicate particles and phosphate buffered saline, combined with a hydrophilic colloid, which forms a tablet or capsule that releases nucleic acids at high temperatures, eliminating the need for additional enzymes or solvents and allowing for standardized extraction across various biological matrices.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional methods using CTAB and organic solvents are used for nucleic acid extraction, then nucleic acids can be isolated from diverse samples, but the process becomes laborious, time-consuming, and requires expensive hazardous chemicals
Solution Approach 1:
The invention extracts and removes the hazardous components (CTAB, organic solvents, enzymes) from the extraction process, retaining only the essential function of nucleic acid isolation. The simplified composition uses safe water-soluble salts and hydrated magnesium silicate particles to achieve the same isolation effect without the laborious steps and dangerous chemicals of conventional methods
Solution Approach 2:
The invention replaces expensive, hazardous chemicals with inexpensive, safe, water-soluble salts and common materials like hydrated magnesium silicate. The simplified composition can be discarded after use without special handling, eliminating the need for costly waste disposal procedures associated with conventional reagents
2Reliability
If conventional chemical treatment methods are used, then nucleic acids can be purified, but the process requires multiple labor-intensive steps and hazardous substances
Solution Approach 1:
The invention merges multiple separate purification steps into a single integrated composition. The water-soluble salts, hydrated magnesium silicate particles, and hydrophilic colloids work together in one mixture to simultaneously perform lysis, purification, and protection functions, eliminating the need for sequential handling of multiple hazardous chemicals
Solution Approach 2:
The simplified composition is designed to be universally effective across diverse sample types (food, biological materials, processed matrices) without requiring method adjustments. The multi-functional mixture handles various sample compositions reliably, making the operation equally simple regardless of sample diversity
3Ease of operation
If standardized extraction methods are applied to diverse food samples, then processing becomes simpler, but the diverse compositions of raw and processed samples require differential treatment
Solution Approach 1:
The invention creates a universal extraction composition that adapts to diverse sample types through its multi-functional design. The water-soluble salts, hydrated magnesium silicate, and hydrophilic colloids collectively handle different sample matrices (raw foods, processed materials, mixed compositions) without requiring method modification, achieving both standardization and versatility
Solution Approach 2:
The invention uses parameters such as pH control through phosphate-buffered saline and temperature-dependent solubility to adapt the extraction process to diverse samples. The composition maintains effectiveness across varying sample compositions by leveraging these physical-chemical parameter changes rather than requiring procedural adjustments
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables rapid, safe, and economically advantageous nucleic acid extraction from food matrices, reducing the need for labor-intensive methods and minimizing health risks associated with conventional chemicals, while ensuring high-quality DNA suitable for PCR analysis.
Implementation Method 1
particles consisting of water-insoluble hydrated magnesium silicate
Implementation Method 2
crystalline phosphate buffered saline which is readily water-soluble to produce a solution having a pH from 5.5 to 7.0
Implementation Method 3
hydrophilic colloid which is effective to disperse the mixture of solids in water
Implementation Method 4
heating the solution or dispersion up to a temperature from 70 to 95 degrees Celsius for 1 minute to 30 minutes, preferably for about 5 minutes to 20 minutes, to release the nucleic acids from the cellular materials
Data Source
AI summary
Composition, method and kit of parts for a simplified and safe extraction of nucleic acids from biological samples, such as crude and processed food, and subsequent analysis by polymerase chain reaction to the presence of animal material, genetically modified organisms, allergens and pathogens. The method comprises the addition of extracting composition together with hot water for extraction and stabilization of nucleic acids as well as removal of substances interfering with DNA polymerase activity.