Direct Amplification Preservation Solution for Nucleic Acid Detection
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Solution Overview
Problem
Current nucleic acid detection methods are cumbersome, time-consuming, and prone to contamination, requiring multiple steps including sample collection, extraction, and storage at low temperatures, which does not meet the demand for rapid and accurate detection.
Innovation Solution
A direct amplification method using a preservation solution containing protective agents, nuclease inhibitors, surfactants, buffer systems, and bacteriostatic agents, combined with ultrasonic treatment, allowing for rapid extraction and detection of nucleic acids without the need for separate extraction steps, and enabling storage at room temperature.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional nucleic acid extraction method is used, then nucleic acid purity and integrity are improved, but detection time and process complexity increase
Solution Approach 1:
The patent combines sample preservation, nucleic acid release, and detection into a single integrated process. The preservation solution contains lysis buffers, protease inhibitors, and other components that simultaneously preserve samples and enable direct nucleic acid amplification without separate extraction steps, reducing total detection time while maintaining quality
Solution Approach 2:
The preservation solution is prepared in advance with all necessary components for nucleic acid preservation and release already included. This preliminary preparation eliminates the need for subsequent extraction steps and allows direct amplification, significantly reducing detection time while ensuring nucleic acid quality through pre-formulated protective components
2Measurement precision
If conventional extraction method is used, then nucleic acid quality is improved, but contamination risk increases
Solution Approach 1:
The patent extracts and removes the problematic intermediate extraction step from the workflow. By using a preservation solution that enables direct amplification, the method eliminates the separate extraction process that creates contamination opportunities, while maintaining nucleic acid quality through the protective formulation
Solution Approach 2:
The preservation solution acts as an intermediary medium that bridges sample collection and detection. It contains protective agents, lysis buffers, and inhibitors that prevent contamination and degradation during the transition from sampling to amplification, ensuring nucleic acid quality without requiring separate extraction
3Stability of the object's composition
If low temperature storage is used, then nucleic acid stability is improved, but storage time and operational complexity increase
Solution Approach 1:
The patent changes the storage parameter from low temperature to room temperature by formulating a preservation solution with stabilizing components. The solution contains buffers, protective agents, and inhibitors that maintain nucleic acid stability at ambient temperatures, eliminating the need for cold chain storage while extending storage time
Solution Approach 2:
The preservation solution is formulated in advance with protective components that cushion against degradation. It contains stabilizing agents and inhibitors that prevent nucleic acid degradation at room temperature, allowing extended storage time without quality loss while simplifying operational requirements
4Ease of operation
If conventional preservation solution is used, then sample storage is simplified, but nucleic acid degradation occurs
Solution Approach 1:
The patent uses a composite preservation solution containing multiple functional components: lysis buffers, protease inhibitors, RNase inhibitors, and other protective agents. This composite formulation simultaneously simplifies storage operations and prevents nucleic acid degradation by addressing multiple degradation pathways through combined protective mechanisms
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method significantly reduces the detection time to less than 1 hour, improves detection efficiency, and ensures the accuracy and reliability of results, while also simplifying the process and reducing contamination risks.
Implementation Method 1
performing ultrasonic treatment to obtain a sample nucleic acid solution
Data Source
AI summary
Disclosed is a nucleic acid detection method, and more particularly a method for rapidly extracting and detecting nucleic acids. The application provides a nucleic acid detection method which includes the following steps: S1, mixing a to-be-tested sample with a direct amplification preservation solution, and performing ultrasonic treatment to obtain a sample nucleic acid solution; S2, adding the sample nucleic acid solution obtained in step S1 into an amplification reaction solution to obtain a mixed solution; S3, carrying out amplification reaction by the mixed solution, and detecting nucleic acids; where the direct amplification preservation solution includes a protective agent, a nuclease inhibitor, a surfactant, a buffer system and a bacteriostatic agent. By the method provided by the application, the whole detection process is simple and less affected, and the detection time is short.