Nucleic Acid Self-Assembled Complex for Stable Raman Detection
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Solution Overview
Problem
Current methods for detecting target nucleic acids using Raman scattering signals face challenges with signal instability and low reproducibility in liquid phases, and the sensitivity to non-specific signals is high, leading to potential incorrect results.
Innovation Solution
A nucleic acid-based self-assembled complex is designed with pre-synthesized metal nanoparticles forming nanogaps that enhance surface plasmon resonance, providing a reproducible Raman scattering signal by interposing Raman indicators in these nanogaps, which are structurally stable and function as a turn-off signal system to quantify target nucleic acids.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If Raman scattering signals are used for detecting target nucleic acids in liquid phase, then the detection method is simple and can measure trace amounts, but the scattering light signal is unstable and shows low reproducibility due to Brownian motion
Solution Approach 1:
The patent changes the physical state parameter of the detection system by transitioning from free-moving nanoparticles in liquid to a solid-supported structured assembly. This parameter change eliminates Brownian motion effects while maintaining the advantages of Raman scattering detection, thereby achieving both operational simplicity and signal reproducibility
Solution Approach 2:
The patent employs a structured assembly with a support structure that provides a fixed platform for the nucleic acid probe and metal nanoparticles. This structural framework acts as a stable matrix that maintains the functional components in fixed positions, eliminating the instability caused by liquid-phase Brownian motion while preserving the detection capabilities
2Measurement precision
If sensitivity to non-specific signals is increased to detect target nucleic acids, then detection sensitivity improves, but false positives increase leading to potential incorrect results
Solution Approach 1:
The patent introduces a specifically designed nucleic acid probe as an intermediary that selectively hybridizes with the target sequence. This probe acts as a mediator that distinguishes between specific and non-specific binding events, enabling high sensitivity detection while maintaining result accuracy through sequence-specific recognition
Solution Approach 2:
The patent applies localized functional properties to different components of the system: the nucleic acid probe provides sequence-specific recognition at the molecular level, while the metal nanoparticles provide localized surface plasmon resonance enhancement at specific spatial locations. This local differentiation of functions enables specific detection with high sensitivity and accuracy
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution achieves stable and reproducible Raman scattering signals in liquid phases, improving the signal-to-noise ratio and enabling accurate quantification of target nucleic acids while minimizing false positives.
Implementation Method 1
forming nanogaps on metal nanoparticle-based structures for generating and further enhancing a surface plasmon resonance phenomenon
Implementation Method 2
Raman spectroscopy is a method of directly irradiating a measurement target sample with light. It is easy to measure, it is possible to measure even a trace amount of sample
Implementation Method 3
Self-assembly has the characteristic of forming a stable structure not by strong bonds such as covalent bonds, but by collective action of relatively weak bonds such as hydrogen bonds, ionic bonds, and van der Waals bonds
Implementation Method 4
Self-assembly refers to formation of a structure by spontaneous non-covalent bonding between components
Implementation Method 5
Hybridization or hybrid probes, used in molecular biology, are DNA or RNA fragments of diverse lengths that can be radioactively or fluorescently labeled, and can be used in DNA- or RNA-containing samples to detect the presence of a nucleotide material
Data Source
AI summary
The present invention relates to a nucleic acid based self-assembled complex for Raman detection of a target nucleic acid, and use thereof. The present invention provides a nucleic acid based self-assembled complex of turn-off based way for detecting a target nucleic acid, which has a structural stability so as produce a reproducible scattering light signal regardless of Brownian motion in liquid and relates to a method of detecting a target nucleic acid as an optical signal such as a Raman signal through a nucleic acid based self-assembled complex performing Brownian motion in a liquid phase.