Nucleic Acid Signal Differentiation via Temperature
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Solution Overview
Problem
Conventional real-time detection technologies cannot differentiate signals from multiple target nucleic acid sequences using a single type of label and detector, requiring multiple labels or melting analysis, which are time-consuming and complex.
Innovation Solution
The method involves detecting signals from target nucleic acid sequences at different temperatures and using mathematical equations to differentiate and assign signals to specific sequences, even when using a single type of label and detector.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If multiple types of labels are used to detect multiple target nucleic acid sequences, then signal differentiation is achieved, but device complexity and cost increase
Solution Approach 1:
The patent changes the detection parameter from label type to detection temperature. By detecting signals at multiple different temperatures (e.g., 60°C and 72°C), the system differentiates between multiple target nucleic acid sequences using a single label type, thus avoiding the complexity of using multiple labels while maintaining signal differentiation capability
2Measurement precision
If melting analysis is used to detect multiple target nucleic acid sequences, then signal differentiation is achieved, but detection time increases
Solution Approach 1:
The patent performs signal differentiation during the real-time detection process itself, rather than requiring a separate melting analysis step. By incorporating temperature-dependent detection into the amplification cycle, the system achieves signal differentiation without adding extra time-consuming steps after the basic detection is complete
3Measurement precision
If multiple types of labels are used for detecting multiple target nucleic acid sequences, then detection accuracy is improved, but cost-effectiveness decreases
Solution Approach 1:
The patent makes a single label type universal by enabling it to detect multiple different target nucleic acid sequences through temperature-dependent differentiation. This multi-functional approach allows one label to replace multiple specialized labels, reducing costs while maintaining the ability to accurately detect various targets
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables efficient, cost-effective, and convenient detection of multiple target nucleic acid sequences using a single label and detector, improving the speed and accuracy of nucleic acid detection.
Implementation Method 1
The real-time detection methods generally use labeled probes or primers specifically hybridized with target nucleic acid sequences
Data Source
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AI summary
The present invention relates to differentiating signals of interest for target nucleic acid sequences. The present invention permits to obtain an individual signal value {i.e., variable) contained in a total signal detected at detection temperatures by using mathematical equations. The present invention based on equation-solving approach enables to obtain the individual signal value in a systematical manner, thereby providing analysis results in much more accurate and convenient manner.