Nucleic Acid Stabilization Reagent for Cell Storage
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Solution Overview
Problem
Current methods for storing biological cells for nucleic acid analysis often result in altered nucleic acid expression due to storage conditions, making it difficult to obtain representative samples of the cell's pre-storage state.
Innovation Solution
A stabilization kit and method using irreversible protein translation inhibitors, ribonucleic acid transcription inhibitors, and electron transport chain agents to stabilize nucleic acids within biological cells, preventing changes during storage and allowing for accurate post-storage analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If biological cells are stored for periods of time before nucleic acid isolation, then storage and logistics are simplified, but nucleic acid expression becomes altered and unrepresentative of the pre-storage state
Solution Approach 1:
The patent applies preliminary action by adding stabilization reagents (translation inhibitors, transcription inhibitors, and electron transport chain agents) to cells before storage. This pre-treatment prevents nucleic acid expression changes from occurring during storage, allowing cells to be stored for extended periods while maintaining representative nucleic acid profiles for later analysis
Solution Approach 2:
The patent changes the biochemical parameters of the cell by introducing specific chemical agents that inhibit translation, transcription, and electron transport. These parameter changes (adding inhibitors at specific concentrations) stabilize the cell's nucleic acid expression state, enabling long-term storage without degradation or alteration of the nucleic acid profile
2Reliability
If stabilization reagents are added to cells, then nucleic acid expression is stabilized during storage, but the complexity of the storage protocol increases
Solution Approach 1:
The patent merges multiple stabilization functions into a single reagent formulation containing translation inhibitors, transcription inhibitors, and electron transport chain agents. This combined approach stabilizes nucleic acid expression through multiple mechanisms simultaneously, simplifying the protocol compared to sequential application of separate agents while maintaining high reliability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution effectively minimizes alterations in nucleic acid expression, ensuring that isolated nucleic acids are representative of the cell's pre-storage state, enhancing the reliability of analysis and storage processes.
Implementation Method 1
at least one irreversible protein translation inhibitor
Implementation Method 2
at least one ribonucleic acid transcription inhibitor
Implementation Method 3
at least one electron transport chain agent chosen from an electron transport chain inhibitor and an electron transport chain decoupling agent
Data Source
AI summary
Reagents for stabilizing the nucleic acids of a biological cell, compositions, kits and methods of use thereof are described. The stabilization reagents may prepare the nucleic acids within the biological cell for storage and preserve the representative population of the nucleic acids for later isolation and analysis.