Nucleic Acid Analysis Substrate With Positioning Markers
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Solution Overview
Problem
Existing nucleic acid analysis methods face challenges with positional deviation during repeated analysis cycles, making it difficult to accurately map DNA fragments and determine base sequences.
Innovation Solution
A substrate with partitioned analysis areas, including absorbent and non-absorbent portions, and a marker portion with a predetermined shape to facilitate precise positioning, combined with a flow cell and nucleic acid analysis device for reproducible analysis area positioning.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If the same analysis area is repeatedly analyzed by sequentially changing analysis areas, then multiple DNA fragments can be analyzed concurrently, but positional deviation occurs in the analysis area at every cycle making it difficult to map DNA fragments accurately
Solution Approach 1:
A marker portion is introduced as an intermediary reference element within the analysis area. This marker serves as a stable mediator that enables accurate positioning and mapping of DNA fragments across multiple analysis cycles, resolving the positional deviation issue while maintaining high productivity
2Productivity
If multiple analysis areas are provided on a single substrate to perform overall analysis, then analysis efficiency is improved, but the complexity of positioning and mapping DNA fragments across areas increases
Solution Approach 1:
The substrate is segmented into multiple distinct analysis areas, each containing its own marker portion. This segmentation allows independent positioning within each area while maintaining overall system efficiency, reducing the complexity of tracking and mapping DNA fragments across the entire substrate
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables reproducible and accurate positioning of analysis areas, allowing for reliable and rapid determination of DNA base sequences even during repeated cycles.
Implementation Method 1
an absorbent portion capable of absorbing a DNA fragment
Implementation Method 2
fluorescence emitted from each DNA fragment is detected to determine the base
Data Source
AI summary
The substrate 100 for use in the analysis of a nucleic acid according to the present invention has multiple analysis areas 12 which are partitioned on a substrate 10, and enables the measurement of the analysis areas 12 while interchanging the analysis areas 12 in turn, said substrate 100 being characterized in that each of the analysis areas 12 consists of an adsorption part 13 onto which a DNA fragment or a carrier having the DNA fragment carried thereon can be adsorbed and a non-adsorption part 14 which is a part outside of the adsorption part 13, and the non-adsorption part 14 has, formed on at least a part thereof, a marker part 15 that has a specified shape and helps to identify the positions of the analysis areas 12.