Integrated Nucleic Acid Testing Device Preventing Aerosol Contamination
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current nucleic acid testing methods using lateral flow test strips face challenges such as aerosol contamination, complex operation, and high costs due to the need for separate nucleic acid amplification and test strip integration.
Innovation Solution
A nucleic acid testing device and method that integrates nucleic acid amplification and lateral flow testing, featuring a base plate with specific chambers and channels that allow for efficient liquid flow and mixing, preventing contamination and simplifying the testing process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If nucleic acid amplification is performed in a separate reaction tube, then the amplification process can be completed, but aerosol contamination occurs during opening of the tube
Solution Approach 1:
The patent integrates the nucleic acid amplification reaction tube with the lateral flow test strip into a single integrated device. The reaction tube is directly connected to the sample pad of the test strip, eliminating the need to open the tube after amplification. This merging of functions prevents aerosol contamination while maintaining testing accuracy.
2Loss of substance
If the reaction system volume is kept small to reduce reagent consumption, then reagent usage is optimized, but the lateral flow test strip requires greater solution volume
Solution Approach 1:
By integrating the amplification reaction tube with the lateral flow test strip, the patent creates a unified system where the reaction volume and test strip fluid volume are coordinated. The integrated design allows the small-volume amplification reaction to directly load the test strip sample pad, eliminating the need for separate dilution steps and optimizing overall reagent usage.
Solution Approach 2:
The test strip sample pad is pre-treated with specific reagents during manufacturing. This preliminary preparation allows the pad to efficiently capture and process the amplification products from the small-volume reaction, reducing the need for additional solution volume during testing.
3Quantity of substance
If a dilution step is added to match solution volumes, then the test strip can be properly loaded, but the overall operation complexity increases
Solution Approach 1:
The integration of the amplification reaction tube with the lateral flow test strip eliminates the need for separate dilution steps. The direct connection allows amplification products to flow directly onto the test strip sample pad, simplifying the操作流程 while maintaining proper solution volumes.
Solution Approach 2:
The patent removes the dilution step from the testing workflow by designing the integrated system to accommodate the natural volume relationship between the amplification reaction and test strip requirements. This extraction of the unnecessary step reduces operational complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enables quick, effective, and cost-efficient nucleic acid amplification and testing, preventing contamination and simplifying operations, making it suitable for grassroots and home testing.
Implementation Method 1
a bent channel that connects the sample chamber and the test strip chamber, and at least one point in the bent channel is higher than a highest liquid level for a solution to be added in the sample reaction chamber
Implementation Method 2
A sample reaction chamber for adding and containing a nucleic acid sample solution and a nucleic acid amplification reagent
Data Source
AI summary
A nucleic acid testing device and method are provided. A base plate that is turned during nucleic acid testing is included, wherein the base plate includes the following chambers: a sample chamber for adding and containing a nucleic acid sample solution, the nucleic acid sample solution is placed in the chamber, and an upper port of the sample chamber communicates with the outside atmosphere; a reaction chamber for nucleic acid amplification reaction and located below the sample chamber, a nucleic acid amplification reagent for nucleic acid amplification reaction is pre-placed in the chamber, and an upper end of the reaction chamber communicates with a bottom end of the sample chamber though a longitudinal liquid channel; a test strip chamber for lateral flow test strip testing, a nucleic acid test strip is pre-placed in the chamber, and the test strip chamber communicates with the reaction chamber through a bent channel.