Nucleic Acid Testing Device Shaped Body Mediator
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Solution Overview
Problem
Existing lateral flow chromatographic devices for nucleic acid detection face challenges in achieving high sensitivity and clear judgment lines due to excessive interaction between reagents and the flow path member, leading to blurring of test and control lines and inefficient use of capture nucleic acid.
Innovation Solution
A testing device with a porous flow path member, a testing target liquid dropping portion, a labeling portion, and a detecting portion, where a capture nucleic acid is immobilized by covalent binding to a shaped body on the flow path member, allowing for controlled affinity and improved immobilization of target nucleic acids.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If capture nucleic acid is directly coated on the flow path member, then the device structure is simple, but the measurement sensitivity is low and judgment lines are blurred
Solution Approach 1:
A shaped body serving as an intermediary carrier is introduced between the flow path member and the capture nucleic acid. The capture nucleic acid is immobilized on the shaped body surface, which is then placed on the flow path member. This intermediary structure prevents direct coating while enabling controlled reagent interaction, thereby improving measurement sensitivity and judgment line clarity without excessive structural complexity.
2Ease of manufacture
If capture nucleic acid is directly coated on the flow path member, then the coating process is simple, but the reagents interact excessively with the flow path member causing blurring
Solution Approach 1:
The shaped body acts as a mediator that simplifies the coating process (capture nucleic acid is coated on the shaped body rather than directly on the flow path member) while simultaneously preventing excessive interaction between reagents and the flow path member. This resolves the contradiction by maintaining ease of manufacture while achieving clear judgment lines.
3Productivity
If capture nucleic acid is immobilized without shaped body, then the device structure is simple, but the utilization of capture nucleic acid is inefficient
Solution Approach 1:
The shaped body serves as an efficient carrier that enhances capture nucleic acid utilization by providing a controlled surface for immobilization and interaction. The intermediary structure optimizes the functional performance of capture nucleic acid while maintaining reasonable device complexity through the use of a relatively simple shaped body component.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enables high-sensitivity measurements with clear judgment lines by optimizing the interaction between reagents and the flow path member, reducing blurring and enhancing the utilization of capture nucleic acid, resulting in improved diagnostic accuracy.
Implementation Method 1
A capture nucleic acid including a sequence bindable and complementary with the target nucleic acid is immobilized by covalent binding to a surface of the shaped body between the shaped body and the flow path member
Implementation Method 2
a detecting portion configured to detect the target nucleic acid labeled at the labeling portion
Data Source
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AI summary
Provided is a testing device including: a porous flow path member constituting a flow path through which a testing target liquid is flowed; a testing target liquid dropping portion provided on the flow path member; a labeling portion configured to apply a label to a target nucleic acid when the target nucleic acid is contained in the testing target liquid dropped onto the testing target liquid dropping portion; and a detecting portion configured to detect the target nucleic acid labeled at the labeling portion, wherein the testing device includes a shaped body formed of a resin on the flow path member at the detecting portion, and wherein a capture nucleic acid including a sequence bindable and complementary with the target nucleic acid is immobilized by covalent binding to a surface of the shaped body between the shaped body and the flow path member.