Light Sheet Microscope Control Through Oblique Image Transformation
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Solution Overview
Problem
Light sheet microscopy produces images that are not intuitively understandable for users due to oblique light sheets, affecting the operation of the device and requiring complex mathematical operations for optimal parameter settings.
Innovation Solution
A method and device for transforming oblique light sheet images from a camera space to a user space, allowing intuitive selection and control of image capture through a user interface, using a transformation matrix to align the image with a single-view focal plane and displaying it in a Cartesian coordinate system.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-affected harmful factors
If oblique light sheets are used to illuminate the sample, then photodamage and photobleaching are minimized, but the resulting image is not intuitively intelligible for users
Solution Approach 1:
The patent introduces an intermediary transformation system that converts oblique light sheet images into a user-friendly coordinate system. A computational module acts as an intermediary, receiving the raw oblique images and transforming them into a corrected coordinate system that is intuitive for users, while preserving the minimal photodamage benefit of oblique illumination.
Solution Approach 2:
The patent applies parameter changes by transforming the coordinate system parameters of the captured images. The oblique light sheet images are mathematically transformed to correct the coordinate distortion, changing the parameter representation from oblique coordinates to a standard Cartesian coordinate system, making the images intuitively intelligible while maintaining the benefits of oblique illumination.
2Measurement precision
If oblique light sheets are used for illumination, then imaging depth and resolution are improved, but complex mathematical operations are required for optimal parameter settings
Solution Approach 1:
The system performs self-service by automatically executing the complex mathematical transformations and coordinate system corrections without requiring user intervention. The computational module autonomously processes the oblique light sheet images, applying the necessary mathematical operations to correct the coordinate system, thereby maintaining high imaging depth and resolution while eliminating the complexity burden on the user.
Solution Approach 2:
The patent replaces manual mechanical adjustment and complex mathematical parameter settings with an automated computational transformation system. Instead of requiring users to perform complex calculations or manual adjustments, the system uses software-based computational methods to automatically correct the coordinate system, simplifying the user interface while maintaining precision.
3Ease of operation
If conventional point-scanning methods are used, then image intuitiveness is maintained, but photodamage and photobleaching increase
Solution Approach 1:
The patent applies segmentation by using thin light sheets to illuminate only specific planes of the sample at a time, rather than illuminating the entire sample volume simultaneously as in conventional point-scanning methods. This segmented illumination approach reduces the total photodamage and photobleaching while maintaining image quality and intuitiveness through computational coordinate transformation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables users to operate the light sheet microscope with conventional 3D visualization, reducing photostress on specimens by allowing intuitive selection and control of image capture without complex mathematical operations.
Implementation Method 1
capturing an image of a volume in a camera space by exciting the volume with one or more light sheets that are oblique relative to an optical axis of a primary lens
Implementation Method 2
transforming the image from the camera space to a user space by mapping information from the one or more light sheet images to one or more single-view focus planes
Data Source
AI summary
A method for operating a light sheet microscope includes capturing an image of a volume in a camera space by exciting the volume with light sheets that are oblique relative to an optical axis of a primary lens of the light sheet microscope and are emitted at different positions in the volume and by capturing a light sheet image for each light sheet, transforming the image from the camera space to a user space by mapping information from the light sheet images to single-view focus planes, displaying the volume in the user space at a user interface, receiving a selection information in the user space about a part of the volume, retransforming the selection information from the user space to the camera space to control the light sheet microscope for capturing a selected image based on the selection information, and capturing the selected image about the part of the volume.