Observation Device with Multi-Angle Imaging for Multiple Scattering
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Solution Overview
Problem
Conventional optical diffraction tomography (ODT) struggles to effectively image three-dimensional cell tissues due to the overwhelming influence of multiple scattered light, leading to speckle generation and deterioration of the single-to-multi-scattering ratio (SMR), making it difficult to extract structural information.
Innovation Solution
An observation apparatus and method that utilizes an interference intensity image acquisition unit, complex amplitude image generation, transmission matrix calculation, and phase differential image generation to reduce the impact of multiple scattered light, enabling clear imaging of multiple scattering objects.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional optical diffraction tomography (ODT) is used to image three-dimensional cell tissues, then three-dimensional structural information can be obtained, but multiple scattered light causes speckle generation and deterioration of the single-to-multi-scattering ratio (SMR), making it difficult to extract structural information
Solution Approach 1:
The patent extracts and utilizes only the singly scattered light component while eliminating the influence of multiply scattered light. By measuring the transmission matrix and applying singular value decomposition, the method separates the useful singly scattered light signal from the harmful multiple scattered light background, thereby improving measurement precision without being affected by speckle generation
Solution Approach 2:
The patent changes the parameter of light scattering order by selectively measuring and processing only singly scattered light. Through parameter optimization in the transmission matrix analysis and applying regularization techniques, the method transforms the problematic multiple scattering situation into a solvable single scattering problem, enabling clear three-dimensional imaging
2Measurement precision
If conventional ODT measures transmission matrix to achieve three-dimensional refractive index tomography, then imaging capability is improved, but the acquired image is merely a two-dimensional projection that cannot grasp true three-dimensional structure
Solution Approach 1:
The patent transitions from two-dimensional projection imaging to three-dimensional tomographic imaging by measuring the transmission matrix from multiple illumination angles and applying inverse scattering algorithms. This dimensional transformation enables reconstruction of the true three-dimensional refractive index distribution, recovering depth information that was lost in conventional two-dimensional projections
3Reliability
If non-staining and non-invasive imaging technique like OCT is used, then observation object is not damaged, but resolution is low and it is difficult to interpret the signal
Solution Approach 1:
The patent changes the imaging parameter from intensity-based measurement (OCT) to phase-based measurement through transmission matrix analysis. By measuring the complex transmission matrix including both amplitude and phase information, and applying singular value decomposition with regularization, the method achieves high-resolution three-dimensional imaging while maintaining non-invasive characteristics
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The approach effectively suppresses speckle and maintains a high single-scattering-to-multi-scattering ratio (SMR), allowing for accurate, non-invasive, and three-dimensional imaging of complex structures like three-dimensional cell tissues.
Implementation Method 1
an interference intensity image acquisition unit for acquiring an interference intensity image of an observation object irradiated with light along each of a plurality of light irradiation directions
Data Source
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AI summary
An observation apparatus 1A includes a light source 11, a mirror 22, a condenser lens 24, an objective lens 25, a beam splitter 41, an imaging unit 43, and an analysis unit 50. The analysis unit 50 irradiates an observation object S with light along each of a plurality of light irradiation directions by changing an orientation of a reflection surface of the mirror 22, acquires an interference intensity image at a reference position for each of the plurality of light irradiation directions from the imaging unit 43, and obtains a phase differential image of the observation object by performing predetermined processing based on the acquired interference intensity images. Thus, an observation apparatus capable of reducing influence of multiple scattered light and easily observing an observation object even in the case in which the observation object is a multiple scattering object is realized.