Oceanobacillus HMF12 Inoculant for Saline Soil and Gray Mold Control

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Solution Overview

Problem

Soil salinization and plant diseases, particularly gray mold caused by Botrytis cinerea, hinder sustainable agriculture by inhibiting plant growth and seed germination, especially in crops like cucumbers, tomatoes, eggplants, and strawberries.

Innovation Solution

The use of Oceanobacillus jeddahense strain HMF12, which is salt-alkali tolerant and antagonistic to Botrytis cinerea, is applied as a microbial inoculant to promote seed germination, enhance plant growth, and control crop diseases under salt stress conditions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If conventional agricultural methods are used, then crop production is maintained, but soil salinization and plant diseases inhibit plant growth and seed germination

Engineering Contradiction:

Improvecrop productionVSAvoidsoil salinization and plant diseases

Core Design Contradiction:

ProductivityVSObject-affected harmful factors

Solution Approach 1:

The patent applies this principle by using salt-alkali tolerant bacteria to convert harmful saline-alkali soil conditions into beneficial environments for crop growth. The bacteria colonize the soil, produce growth-promoting substances, and enhance plant immunity, transforming the previously harmful salinity stress into a condition that supports both disease control and plant development.

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The patent employs salt-alkali tolerant bacteria as intermediary agents between the harmful soil environment and the crops. These bacteria mediate the interaction by producing protective substances, enhancing plant root development, and stimulating immune responses, thereby protecting plants from both salinity stress and pathogenic infections.

Inventive Principle:

Principle #24Intermediary (Mediator)

2Reliability

If chemical pesticides and fertilizers are used, then disease control and growth promotion are achieved, but environmental pollution and resistance development occur

Engineering Contradiction:

Improvedisease controlVSAvoidenvironmental pollution and resistance

Core Design Contradiction:

ReliabilityVSObject-generated harmful factors

Solution Approach 1:

The patent applies self-service by utilizing the plant's own immune system and growth mechanisms, enhanced by bacterial-produced substances. The bacteria stimulate the plant's endogenous defense responses and growth hormones, allowing the plant to protect itself and promote growth through its own physiological processes rather than external chemical interventions.

Inventive Principle:

Principle #25Self-service

Solution Approach 2:

The patent changes the biological parameters of the plant system by introducing beneficial bacteria that modify plant physiology. The bacteria alter plant hormone levels, enhance immune gene expression, and modify root architecture, thereby changing the plant's biological parameters to achieve disease resistance and growth promotion without chemical pesticides.

Inventive Principle:

Principle #35Parameter changes

3Adaptability or versatility

If salt-alkali tolerant microorganisms are introduced, then colonization rate increases, but disease control efficacy must be maintained

Engineering Contradiction:

Improvesalt-alkali toleranceVSAvoiddisease control efficacy

Core Design Contradiction:

Adaptability or versatilityVSReliability

Solution Approach 1:

The patent applies universality by selecting and utilizing salt-alkali tolerant bacteria that perform multiple functions simultaneously. These bacteria both tolerate saline-alkali environmental conditions and provide disease control services through production of antimicrobial substances and stimulation of plant immunity, achieving multi-functionality that resolves the contradiction between adaptability and reliability.

Inventive Principle:

Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Oceanobacillus jeddahense strain HMF12 demonstrates strong salt-alkali tolerance, improves crop emergence rates, promotes growth, and effectively controls gray mold with an efficacy of 86.45% against strawberry gray mold, showing better colonization and disease control compared to conventional methods.

Implementation Method 1

The Oceanobacillus jeddahense strain HMF12 has strong salt-alkali tolerance and high colonization rate in saline-alkali soil. The microbial inoculant prepared using this strain can improve crop emergence rates under saline-alkali soil conditions, promote crop growth, and prevent and control crop gray mold caused by Botrytis cinerea

Methodology Applied

Scientific EffectAntibiotic production:

Implementation Method 2

the Oceanobacillus jeddahense strain HMF12 according to the invention has strong salt-alkali tolerance and high colonization rate in saline-alkali soil

Methodology Applied

Scientific EffectColonization:

Data Source

PatentUS12584102B2<i>Oceanobacillus jeddahense </i>strain HMF12 and its application

Publication Date: 2026.03.24 GUO YANG INTL MICROBIOLOGY GROUP

AI summary

The invention relates to an Oceanobacillus jeddahense strain HMF12, preservation number CGMCC No. 30478. The microbial inoculant contains 4×109 cfu/g to 7×109 cfu/g of Oceanobacillus jeddahense HMF12 spores. This strain can promote seed germination and plant growth under salt stress conditions and effectively prevent and control crop diseases, especially strawberry gray mold.