OG CITRON Cannabis Cultivar Stabilization via Cloning

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Solution Overview

Problem

The Cannabis genus has a complex taxonomy and nomenclature, making it difficult to distinguish between species and cultivars, particularly due to the promiscuous crosses in indoor breeding programs, which affects the development of cultivars with specific medicinal and psychoactive effects.

Innovation Solution

The development of a new Cannabis cultivar, 'OG CITRON', through controlled-crosses and asexual reproduction via stem cutting and cloning, which stabilizes the unique blend of cannabinoids and terpenes for enhanced medicinal, psychoactive, and recreational effects.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If controlled-crosses and asexual reproduction are used, then cultivar stability and true-to-type reproduction are improved, but breeding program complexity increases

Engineering Contradiction:

Improvecultivar stabilityVSAvoidbreeding program complexity

Core Design Contradiction:

Stability of the object's compositionVSDevice complexity

Solution Approach 1:

The patent employs asexual reproduction through stem cuttings and cloning to create exact genetic copies of the parent plant. This copying process ensures that the unique cannabinoid and terpene profile of 'OG CITRON' is replicated true-to-type across multiple generations, maintaining cultivar stability without requiring complex genetic manipulation procedures

Inventive Principle:

Principle #26Copying

Solution Approach 2:

The patent performs controlled-crosses between selected parent plants before asexual reproduction to establish the desired genetic combination. This preliminary sexual crossing phase allows breeders to create the optimal hybrid genotype, which is then fixed and stabilized through subsequent asexual propagation, separating the complexity of genetic selection from cultivar multiplication

Inventive Principle:

Principle #10Preliminary action

2Adaptability or versatility

If promiscuous crosses occur in indoor breeding programs, then genetic diversity increases, but cultivar distinction and specificity are lost

Engineering Contradiction:

Improvegenetic diversityVSAvoidcultivar distinction

Core Design Contradiction:

Adaptability or versatilityVSMeasurement precision

Solution Approach 1:

The patent divides the breeding process into distinct sequential phases: first, controlled sexual crosses between specific parent plants to generate genetic diversity and create novel combinations; second, rigorous selection and evaluation of progeny to identify desired traits; third, asexual reproduction to fix and propagate the selected genotype. This segmentation prevents premature mixing of unrelated lines and maintains clear cultivar boundaries

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The patent implements continuous monitoring and evaluation of cannabinoid content (THC, CBD, CBG), terpene profiles, plant morphology, and yield characteristics during the breeding program. This feedback mechanism allows breeders to track genetic changes, ensure cultivar uniformity, and make informed decisions about which progeny to propagate asexually, thereby maintaining cultivar distinction while utilizing genetic diversity

Inventive Principle:

Principle #23Feedback

Data Source

PatentUSPP33000P3<i>Cannabis </i>plant named ‘OG CITRON’

Publication Date: 2021.04.27 BIOTECH INSTITUTE LLC

AI summary

Disclosed herein are main terpenes of ‘OG CITRON’, which are linalool, limonene, myrcene, beta-caryophyllene, alpha-bisabolol, alpha-humulene, and beta-pinene. Also, the present invention provides the estimated concentration of the THCmax, CBDmax, and CBGmax, about 9.90-12.66%, about 5.50-9.23%, and about 0.08-0.78%, respectively, at the time of assaying metabolites from flower samples of ‘OG CITRON’.