Oleandrin Extraction via Subcritical Liquid and Organic Solvent Methods
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Solution Overview
Problem
Current methods for producing oleandrin, a compound with therapeutic properties, face challenges in achieving high yields and optimal compositional profiles, particularly in extracting oleandrin from Nerium oleander, with existing processes like supercritical fluid extraction yielding low oleandrin content and varying therapeutic activity profiles.
Innovation Solution
The implementation of subcritical liquid extraction (SbCLE) and organic solvent extraction methods to produce oleandrin-containing extracts, combining these to create a dual extract composition with enhanced oleandrin content and a broader range of phytochemicals, thereby improving yield and therapeutic efficacy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If supercritical fluid extraction is used to produce oleandrin-containing extract, then extraction efficiency is improved, but oleandrin content and therapeutic activity are reduced
Solution Approach 1:
The patent changes the extraction parameters by using subcritical liquid extraction conditions (lower temperature and pressure) instead of supercritical fluid extraction. This parameter change allows for higher oleandrin content (0.5-5% w/w) while maintaining extraction efficiency, resolving the contradiction between extraction efficiency and oleandrin content
Solution Approach 2:
The patent creates a composite extract containing multiple phytochemicals (oleandrin, oleanolic acid, ursolic acid, betulinic acid, kanerocin, kanerodione) with specific compositional ratios. This composite approach ensures both high extraction efficiency and optimal therapeutic activity profile with oleandrin as the predominant cardiac glycoside
2Ease of manufacture
If traditional extraction methods are used, then process simplicity is maintained, but oleandrin yield and compositional profile are suboptimal
Solution Approach 1:
The patent modifies traditional extraction parameters by controlling temperature (30-50°C) and pressure (73-300 atm) to achieve subcritical liquid extraction conditions. This parameter optimization maintains relative process simplicity while dramatically improving oleandrin yield (0.5-5% w/w) and compositional quality
Solution Approach 2:
The patent uses carbon dioxide as an intermediary solvent that can be easily controlled and removed. The CO2 acts as a mediator between the plant material and the extraction process, allowing for simple yet efficient extraction with optimal oleandrin recovery and clean product isolation
3Reliability
If extraction conditions are optimized for high oleandrin content, then therapeutic efficacy is improved, but extraction complexity increases
Solution Approach 1:
The patent optimizes extraction parameters (temperature 30-50°C, pressure 73-300 atm, extraction time 1-24 hours) to achieve high oleandrin content (0.5-5% w/w) and consistent therapeutic efficacy. These parameter changes provide reliable therapeutic results while using standard extraction equipment, avoiding excessive complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach results in higher oleandrin yields and a more favorable compositional profile compared to traditional methods, enhancing the therapeutic effectiveness of oleandrin-containing compositions for treating cardiac glycoside-responsive diseases and disorders.
Implementation Method 1
The invention provides a method of producing oleandrin or oleandrin-containing extract from oleandrin-containing biomass by a) subcritical liquid (SbCL) extraction (SbCLE) with carbon dioxide
Implementation Method 2
b) extraction with organic solvent (OSE), optionally further comprising water
Data Source
AI summary
A subcritical liquid extraction process for the production of oleandrin or oleandrin-containing extract is provided. The process provides improvements over other methods of preparing oleandrin-containing extract. The extract, pharmaceutical compositions, dosage forms and nutraceutical compositions comprising oleandrin or said extract are provided. Methods of treating diseases, disorders or conditions that are therapeutically responsive to oleandrin are also provided.