Olfactory Receptor Gene Diagnostic Kit for Metabolic Disease Screening
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current research lacks effective molecular targets for obesity and metabolic diseases related to lipid metabolism disorders, particularly in peripheral tissues, where olfactory receptors' role in obesity remains unknown.
Innovation Solution
Development of a kit using primers or probes specifically hybridized with nucleotide sequences of olfactory receptor genes expressed in peripheral tissues, such as fat and muscle, to diagnose metabolic diseases like dyslipidemia, fatty liver, and insulin resistance syndrome, and to screen therapeutic compositions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If olfactory receptor genes are used as diagnostic targets for metabolic diseases, then diagnostic capability is improved, but the complexity of the diagnostic system increases
Solution Approach 1:
The patent extracts and isolates specific olfactory receptor gene sequences (such as OR51G2, OR2M1, OR10G4) from the complex genomic background to create targeted diagnostic probes and primers. This extraction approach enables specific detection of metabolic disease markers without analyzing the entire genome, thereby improving diagnostic precision while managing system complexity through focused targeting.
Solution Approach 2:
The patent develops a universal diagnostic platform that can detect multiple different olfactory receptor gene expressions using a common kit structure. The same basic diagnostic system can be applied to detect various metabolic diseases (obesity, diabetes, dyslipidemia) by simply changing the targeted gene probes, providing multi-functionality that improves diagnostic capability across different conditions without proportionally increasing system complexity.
2Measurement precision
If specific olfactory receptor gene probes are developed for each metabolic disease, then diagnostic precision is improved, but the cost and complexity of the kit increase
Solution Approach 1:
The patent segments the diagnostic process into modular components: universal kit infrastructure (reaction buffers, enzymes, detection systems) and disease-specific gene probes. This segmentation allows the complex task of metabolic disease diagnosis to be divided into reusable standard components and variable disease-specific elements, improving precision through targeted probes while controlling overall kit complexity through modular design.
Solution Approach 2:
The patent changes the parameter of gene sequence specificity in the probes while maintaining constant other diagnostic parameters (reaction conditions, detection methods, kit structure). By varying only the targeted gene sequences (OR51G2 for obesity, OR2M1 for diabetes, OR10G4 for dyslipidemia) within a standardized platform, the patent achieves disease-specific precision without proportionally increasing overall kit complexity.
3Reliability
If olfactory receptor gene expressions are targeted in peripheral tissues, then relevance to metabolic diseases is improved, but the difficulty of detecting and measuring increases
Solution Approach 1:
The patent uses PCR amplification as an intermediary step between the low-abundance olfactory receptor gene transcripts in peripheral tissues and the detection system. The PCR process amplifies the target gene sequences millions of times, making them detectable despite their low initial concentration in fat, muscle, or liver tissues, thereby maintaining reliability while reducing detection difficulty.
Solution Approach 2:
The patent performs preliminary RNA extraction and cDNA synthesis from peripheral tissue samples before the actual gene expression analysis. This preliminary preparation concentrates and stabilizes the target molecules, making subsequent detection of olfactory receptor gene expressions (OR51G2, OR2M1, OR10G4) more reliable and less difficult by preparing the samples in advance under optimized conditions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The kit enables reliable diagnosis and screening of metabolic diseases by targeting olfactory receptor gene expressions in peripheral tissues, providing novel gene targets for obesity and related conditions, facilitating the identification of therapeutic candidates.
Implementation Method 1
a kit for diagnosing a metabolic disease selected from a group consisting of dyslipidemia, fatty liver and insulin resistance syndrome, comprising a primer or a probe which is specifically hybridized with the nucleotide sequence
Data Source
AI summary
The present invention relates to a diagnostic kit for metabolic diseases selected from the group consisting of obesity, dyslipidemia, fatty liver, and insulin resistance syndrome, and a method for screening a therapeutic composition for said diseases. The invention provides a large number of new gene targets for metabolic diseases such as obesity and the like, thereby enabling a more reliable diagnosis of genes such as obesity and the like and the use in the screening of a therapeutic candidate material based on the new gene targets.