Oligonucleotide Security Markers for Mass Production
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Solution Overview
Problem
Existing security markers using nucleic acid molecules are costly and inefficient due to low production scale and require specialized laboratory analysis, limiting their application and market potential, and are prone to errors in detection.
Innovation Solution
A security marker system utilizing a pool of oligonucleotides with varying nucleotide sequences, where each combination generates a unique DNA code detectable through PCR and melt curve analysis, allowing for mass production and efficient detection using portable equipment, reducing costs and increasing reliability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a single oligonucleotide with binding sites for a primer pair and a uniquely coded central region is used, then unique identification is achieved, but production efficiency is low and cost is high
Solution Approach 1:
The invention divides the identification system into two parts: a pool of short oligonucleotide sequences (markers) and a detection system using probes and PCR. Instead of producing full-length unique oligonucleotides, the system uses combinations of shorter sequences from a pool, achieving unique identification through combinatorial diversity while enabling mass production of the individual pool components.
2Measurement precision
If a single oligonucleotide with unique sequence is used, then identification accuracy is improved, but analysis requires specialized laboratory equipment and knowledge
Solution Approach 1:
The invention introduces molecular probes as intermediaries that hybridize to the target oligonucleotide sequences. These probes are designed with specific binding characteristics that allow detection through PCR amplification and melt curve analysis, transforming the detection process into a more accessible format that can be performed with standard laboratory equipment rather than requiring specialized forensic analysis facilities.
3Adaptability or versatility
If oligonucleotides with random nucleic acid sequences are used, then unique codes can be generated, but detection is prone to errors
Solution Approach 1:
The invention optimizes the parameters of the oligonucleotide sequences, including length, composition, and melting temperature characteristics. By carefully designing these parameters and using probes with specific binding affinities, the system achieves reliable detection through melt curve analysis, where the melting temperature provides an additional parameter for verification and error reduction.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system enables cost-effective mass production and efficient detection of security markers, reducing errors and expanding their application by using a pool of oligonucleotides to generate unique codes, which can be analyzed quickly and reliably with portable equipment, enhancing their market potential and forensic reliability.
Implementation Method 1
the probe including a predetermined probe nucleotide sequence, the security marker comprising a target oligonucleotide, the target oligonucleotide comprising a pair of primer regions and a marker region located between the primer regions, the marker region comprising a predetermined marker nucleotide sequence which is fully or partially complementary to the probe nucleotide sequence
Implementation Method 2
The use of polymerase chain reaction (PCR) to isolate and multiply a sample of nucleic acid within a security marker is known
Implementation Method 3
Analysis must be performed within a forensic laboratory, with specialist equipment and knowledge of the sample to be tested
Data Source
AI summary
A security marker for cooperation with a detector, the detector including a molecular probe, the probe including a predetermined probe nucleotide sequence. The security marker comprises a target oligonucleotide. The target oligonucleotide comprises a pair of primer regions and a marker region located between the primer regions. The marker region comprises a predetermined marker nucleotide sequence which is fully or partially complementary to the probe nucleotide sequence.