Olive Leaf Nrf2 Activator via Pressurized Hot Water Extraction
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Solution Overview
Problem
There is a strong demand for a novel Nrf2 activator derived from natural components with high activation ability and low cytotoxicity for treating conditions such as Alzheimer's disease and other oxidative stress-related conditions.
Innovation Solution
A pressurized hot water extract of olive leaves is used to produce an Nrf2 activator through extraction at temperatures of 150°C or higher and pressures between 0.1 MPa and 5.0 MPa for 120 minutes or less, maintaining low cytotoxicity while achieving high Nrf2 activation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional extraction methods (hot water or ethanol extraction) are used, then the extraction process is simple, but the Nrf2 activation ability is insufficient
Solution Approach 1:
The patent applies parameter changes by using pressurized hot water extraction at temperatures of 150°C or higher and pressures greater than 0.1 MPa, which are different from conventional extraction conditions. This change in extraction parameters enables the obtainment of an extract with extremely high Nrf2 activation ability while maintaining low cytotoxicity, resolving the contradiction between activation ability and process complexity.
2Productivity
If extraction temperature is increased to improve extraction efficiency, then extraction speed increases, but cytotoxicity may increase
Solution Approach 1:
The patent utilizes parameter changes by operating at temperatures of 150°C or higher under pressurized conditions (greater than 0.1 MPa). The pressurized environment allows high-temperature extraction without causing cytotoxicity, as the pressure prevents water from becoming too aggressive. This resolves the contradiction between extraction efficiency and cytotoxicity by enabling high-temperature extraction in a controlled pressurized environment.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The extract demonstrates an extremely high Nrf2 activation ability compared to conventional methods, with low cytotoxicity, effectively treating conditions like diabetes, inflammation, and skin disorders, and has applications in pharmaceuticals, cosmetics, and food products.
Implementation Method 1
an extract obtained by subjecting olive to pressurized hot water extraction has an unexpectedly high Nrf2 activation ability
Implementation Method 2
The graphs show Nrf2 activation (upper row) and cell viability (lower row) of a pressurized hot water extract of olive leaves (left), a 80% ethanol extract (center), and a hot water extract (right)
Data Source
AI summary
The present invention provides a novel Nrf2 activator that is derived from a natural component and that has a high Nrf2 activation ability. The invention also provides a novel treatment agent for a condition treatable by Nrf2 activation, the treatment agent being derived from a natural component having a high Nrf2 activation ability. Specifically, provided are a treatment agent for a condition treatable by Nrf2 activation, the treatment agent comprising a pressurized hot water extract of olive, and an Nrf2 activator comprising a pressurized hot water extract of olive.