Saccharomyces sp. OMK-58 Fermentation for Natural Tyrosol Production
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Solution Overview
Problem
Current methods for producing tyrosol, such as chemical synthesis and plant extraction, face challenges like environmental pollution, harsh conditions, low yield, and inability to meet market demand due to low tyrosol content in plants and complex extraction processes, while biosynthesis methods have yielded only mg/L levels, which are not industrially viable.
Innovation Solution
A microbial fermentation method using the yeast Saccharomyces sp. OMK-58 strain, which converts tyrosine into natural tyrosol, offering a safe, environmentally friendly, and efficient production process at low temperature and pressure with a yield of up to 5.0 g/L.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If chemical synthesis method is used, then production capacity can be increased, but environmental pollution increases and process conditions become harsher
Solution Approach 1:
The patent replaces chemical synthesis methods with biological fermentation using engineered yeast strains. The metabolic pathway is substituted from chemical reactions to biological metabolism, where the yeast naturally converts substrates to tyrosol through enzymatic reactions, eliminating harsh chemicals and reducing environmental pollution while maintaining production capacity.
Solution Approach 2:
The patent optimizes fermentation parameters including pH (5.0-8.0), temperature (30-37°C), aeration ratio (1:0.05-0.15), and rotation speed (200-500 rpm) to maximize tyrosol yield. By adjusting these biological process parameters, the system achieves g/L level production without the environmental harm of chemical methods.
2Reliability
If plant extraction method is used, then natural tyrosol can be obtained, but yield is low and extraction process is complex
Solution Approach 1:
The engineered yeast strain performs self-service by autonomously converting substrates to tyrosol through its metabolic pathway. The biological system self-regulates the conversion process without requiring complex extraction procedures, naturally producing high concentrations of tyrosol that can be directly harvested from the fermentation broth.
Solution Approach 2:
The patent transforms the production approach from extraction (removing tyrosol from plants) to synthesis (producing tyrosol through fermentation). By changing the fundamental process parameter from extraction efficiency to fermentation yield, the system achieves both natural product status and high production capacity at g/L levels.
3Reliability
If traditional biosynthesis methods are used, then natural tyrosol production is achieved, but yield remains at mg/L level which is not industrially viable
Solution Approach 1:
The patent segments the metabolic pathway by introducing specific gene sequences (including tyrosine ammonia-lyase gene) into the yeast genome. This genetic segmentation enables the yeast to efficiently convert tyrosine to tyrosol through a dedicated enzymatic pathway, achieving yield enhancement from mg/L to g/L levels while maintaining natural product status.
Solution Approach 2:
The patent creates a composite biological system by combining engineered yeast strains with optimized fermentation media containing specific substrates (tyrosine, sugar raw materials, ammonium sulfate, magnesium sulfate, potassium dihydrogen phosphate, yeast extract powder). This composite approach synergistically enhances production yield to industrially viable levels.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method provides a scalable and environmentally friendly production of natural tyrosol with higher yields compared to previous biosynthesis methods, meeting industrial demands and aligning with the growing market preference for natural products.
Implementation Method 1
The yeast Saccharomyces sp. OMK-58 strain activation; 2) the seed culture; 3) the fermentation: the seed liquid cultured to logarithmic growth phase is inoculated into a fermentation culture medium
Data Source
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AI summary
The invention discloses a strain for the production of natural tyrosol and a preparation method for natural tyrosol. The strain is a yeast, Saccharomyces sp. OMK-58. The strain can be used to produce natural tyrosol. The preparation method of natural tyrosol is to use the strain to produce tyrosol by microbial fermentation. The fermentation method includes steps of strain activation, seed culture, fermentation, extraction and so on. The method has the advantages of low temperature and low pressure, relatively safe and simple operation, and less pollution. The present method is a safe and environmentally friendly production method.