One-Pot Biosensor Using Photocatalyst Substrate for Single-Step Detection
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Solution Overview
Problem
Current diagnostic technologies require complex processes and expert handling, making them unsuitable for direct, portable, and simple detection of target molecules by individuals, particularly for point-of-care applications.
Innovation Solution
A portable one-pot biosensor incorporating a photocatalyst substrate with metal nanoparticles and a reaction pad containing a binding material-fluorescent material complex, which generates peroxide upon UV irradiation, enabling specific detection of target molecules through fluorescence resonance energy transfer (FRET) without the need for multiple solution injections or extensive equipment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional diagnostic technology is used, then detection accuracy can be achieved, but the process becomes complicated and requires expert handling
Solution Approach 1:
The patent combines multiple functional components (binding material, fluorescent material, peroxide generation system, and detection mechanism) into a single integrated reaction pad structure. This merging allows the complex diagnostic function to be achieved within one simple device that individuals can operate without expert knowledge, resolving the contradiction between detection accuracy and process complexity
Solution Approach 2:
The reaction pad is designed as a universal platform that integrates sample reception, binding reaction, fluorescent labeling, peroxide generation, and detection signal generation into a single multi-functional component. This universal design enables accurate detection while eliminating the need for separate complex equipment and expert operations
2Measurement precision
If multiple solution injections are performed for detection, then detection accuracy improves, but operation time and complexity increase
Solution Approach 1:
The patent merges the binding reaction and fluorescent labeling steps into a single simultaneous process within the reaction pad. The binding material and fluorescent material are pre-combined in the pad, allowing both functions to occur in one solution injection rather than requiring separate injection steps, thereby reducing operation time while maintaining detection accuracy
Solution Approach 2:
The reaction pad is prepared in advance with the binding material and fluorescent material already combined and positioned. This preliminary preparation eliminates the need for multiple sequential injections during operation, as all necessary components are ready to react simultaneously when the sample is introduced, reducing operation time while preserving detection accuracy
3Measurement precision
If conventional biosensor systems are used, then detection capability is achieved, but portability is limited due to equipment requirements
Solution Approach 1:
The patent extracts the essential detection function from complex conventional biosensor systems and concentrates it into a single portable reaction pad. By removing unnecessary external equipment and retaining only the core functional elements (binding material, fluorescent material, and peroxide generation system integrated in the pad), the system achieves both detection capability and portability for individual use
Solution Approach 2:
The reaction pad is designed to perform all detection functions autonomously without requiring external complex equipment. The peroxide generation system within the pad automatically provides the necessary oxidizing agent, and the fluorescent signal is generated and detected within the pad itself, enabling the system to serve itself and eliminating the need for bulky external equipment, thus achieving portability while maintaining detection capability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The biosensor allows for efficient detection of target molecules by a single solution injection, providing a portable and user-friendly solution for health management, capable of quantifying target concentrations with high sensitivity and specificity, similar to commercial pregnancy diagnostic kits.
Implementation Method 1
the photocatalyst substrate (110) deposited with metal nanoparticles; and a reaction pad (10) which is disposed on an upper surface of the photocatalyst substrate and includes a first binding material-fluorescent material complex specifically binding to a molecule to be detected
Implementation Method 2
a first binding material-fluorescent material complex specifically binding to a molecule to be detected
Data Source
AI summary
Disclosed are a one-pot biosensor and an immunoassay method using the same. The one-pot biosensor includes a photocatalyst substrate deposited with metal nanoparticles; and a reaction pad which is disposed on an upper surface of the photocatalyst substrate and includes a first binding material-fluorescent material complex specifically binding to a molecule to be detected, and the immunoassay method using the same. The one-pot biosensor may detect a target by once solution injection and has a size enough to be portable. Accordingly, since the one-pot biosensor can detect the target by only once solution injection without a washing step, because of a sensor platform capable of being easily used by an individual other than a diagnostic expert, it is predicted to be positioned as a means capable of confirming the health condition of the individual without seeing the doctor, such as a pregnancy diagnostic kit which has been currently commercialized.