Open Channel Solid Phase Extraction Capillaries for Biomolecule Recovery
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for solid phase extractions in open channels face challenges in efficiently purifying and concentrating low-abundance proteins and biomolecules due to high levels of contaminating proteins and complex protein expression dynamics, leading to sample loss and inefficiencies in analytical techniques like mass spectrometry.
Innovation Solution
The use of open channel solid phase extraction capillaries with a bound extraction surface that allows for multiple passes of sample solutions and desorption solvents, minimizing unswept volumes and enabling effective adsorption, elution, and concentration of biomolecules without shearing or filtration issues, using techniques such as covalent attachment of latex particles and specific affinity binding agents.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If packed columns and cartridges containing solid phase are used for protein extraction, then purification and concentration of biomolecules can be achieved, but sample loss occurs and low-abundance proteins are difficult to recover due to high levels of contaminating proteins
Solution Approach 1:
The packed column is segmented into multiple smaller extraction capillaries arranged in parallel. Each capillary processes a portion of the sample independently, reducing the overwhelming effect of contaminating proteins on any single extraction unit and improving recovery of low-abundance proteins through distributed processing
Solution Approach 2:
The solid phase extraction material is taken out from the traditional packed column format and transferred to the inner surface of open capillaries. This extraction surface is formed by coating the capillary interior with latex particles containing affinity ligands, enabling efficient adsorption of target proteins while minimizing sample loss
2Productivity
If traditional packed columns are used for solid phase extraction, then purification can be performed, but unswept volumes and dead spaces cause inefficiencies and sample loss
Solution Approach 1:
The extraction system is segmented into multiple narrow-bore capillaries with open interiors. This segmentation eliminates the dead spaces and unswept volumes inherent in packed columns, as each capillary provides a continuous flow path where all sample contacts the extraction surface, improving extraction efficiency and reducing sample loss
Solution Approach 2:
The capillary inner surfaces are coated with porous latex particles that provide high surface area for protein adsorption. The porous structure of the coating material enables efficient mass transfer while the open capillary geometry ensures complete sample sweeping, eliminating dead volumes
3Measurement precision
If affinity methods are used for enrichment of specific proteins, then purification of low-abundance proteins can be achieved, but the methods are specific for particular functionalities and require integration into miniaturized systems
Solution Approach 1:
The capillary array system provides a universal platform that can be configured with different affinity ligands on the capillary surfaces to enrich various types of proteins. The same basic capillary structure and operation procedure can be applied regardless of the specific target protein, enabling multi-functionality while maintaining simplicity
Solution Approach 2:
The system achieves high enrichment factors by changing physical parameters such as capillary dimensions (narrow bore), flow rates, and coating characteristics. These parameter changes enhance the efficiency of affinity binding while maintaining compatibility with standard analytical instruments, reducing integration complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach minimizes sample loss, enhances the recovery of low-abundance proteins, and achieves high enrichment factors, allowing for the analysis of proteins that would otherwise be undetectable, while maintaining the structural and functional integrity of the biomolecules.
Implementation Method 1
passing them through a packed column and cartridge containing a solid phase where the molecules of interest are adsorbed
Implementation Method 2
specific affinity binding agents
Implementation Method 3
a solvent is used to desorb the molecules of interest and form a concentrated solution
Data Source
AI summary
The invention provides, inter alia, capillary extraction devices, and methods of making and using the same.