Open Substrate Analyte Detection with Immersion Optics
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Solution Overview
Problem
Biological sample processing systems face inefficiencies, being time-intensive and wasteful of valuable resources such as reagents, necessitating the need for high-efficiency methods and systems for sample processing and analysis.
Innovation Solution
The use of an open substrate with immobilized analytes, coupled with an immersion optics system and spatial indexing, allows for efficient scanning and detection of analytes through controlled environments and advanced scanning techniques, including rotational and translational movements of the substrate and scanning field, to facilitate reactions and detections.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional biological sample processing systems are used, then standard analysis can be performed, but processing time is excessive and reagent consumption is high
Solution Approach 1:
The substrate is divided into multiple discrete locations or regions, each capable of holding different analytes or performing different functions. This segmentation allows parallel processing of multiple samples simultaneously, dramatically increasing throughput and reducing overall processing time while minimizing reagent requirements per sample.
Solution Approach 2:
The system transitions from traditional linear or batch processing to a two-dimensional array-based substrate configuration. By organizing analytes across multiple spatial locations on the substrate, the system enables simultaneous multi-point analysis, effectively adding a spatial dimension to the processing workflow and achieving parallelization that reduces both time and reagent consumption.
2Productivity
If conventional biological sample processing systems are used, then standard analysis can be performed, but reagent consumption is excessive
Solution Approach 1:
Reagents are delivered locally to specific regions or locations on the substrate rather than flooding the entire system. This localized delivery approach ensures that reagents are applied only where needed, minimizing waste and reducing overall consumption while maintaining processing efficiency across multiple sample locations.
Solution Approach 2:
The substrate is divided into multiple discrete locations or regions, each capable of holding different analytes or performing different functions. This segmentation allows parallel processing of multiple samples simultaneously, dramatically increasing throughput and reducing overall processing time while minimizing reagent requirements per sample.
3Measurement precision
If scanning field orientation is not maintained during rotation, then scanning complexity is reduced, but detection accuracy deteriorates
Solution Approach 1:
The system incorporates feedback mechanisms that continuously monitor the orientation and position of the scanning field relative to the substrate during rotation. This feedback enables real-time adjustments to maintain proper alignment, ensuring detection accuracy is preserved throughout the scanning process despite the rotational motion and system complexity.
Data Source
AI summary
Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.