Open Substrate Analyte Detection with Immersion Optics
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Biological sample processing systems and methods are often inefficient and wasteful of valuable resources, such as reagents, requiring improvements in efficiency and resource utilization.
Innovation Solution
The use of an open substrate with immobilized analytes and an immersion optics system for scanning and detection, combined with spatial indexing and controlled environments, allows for efficient processing and analysis by facilitating reactions and detections through precise scanning and illumination techniques.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional biological sample processing systems are used, then basic detection functions are provided, but processing efficiency is low and resource consumption is high
Solution Approach 1:
The system segments the substrate into multiple individually addressable locations, allowing selective processing and detection at specific positions. This enables parallel processing of multiple samples or reactions simultaneously, improving productivity while reducing overall reagent consumption by treating only the necessary locations rather than the entire substrate uniformly.
Solution Approach 2:
The invention implements local quality control by providing controlled environments at individual substrate locations, with separate reagent delivery and environmental control for each addressable position. This allows optimization of conditions locally at each position, improving reaction efficiency and reducing waste by applying reagents only where needed rather than across the entire substrate.
2Loss of time
If conventional processing methods are used, then basic analysis is performed, but processing time is excessive
Solution Approach 1:
The system enables continuous processing by allowing multiple operations to occur simultaneously at different substrate locations - different reactions can proceed in parallel at different addressable positions, and the detection system continuously monitors multiple positions without requiring sequential processing. This dramatically reduces total processing time while maintaining high productivity.
Solution Approach 2:
The invention allows preliminary setup of multiple reaction conditions and sample preparations at different substrate locations before actual processing begins. Samples can be pre-positioned, reagents can be pre-loaded into reservoirs, and environmental conditions can be pre-configured, enabling immediate start of parallel processing operations and reducing overall processing time.
3Area of stationary object
If scanning systems rotate the substrate for imaging, then complete surface coverage is achieved, but orientation distortion occurs
Solution Approach 1:
The system dynamically adjusts the scanning pattern to compensate for substrate rotation. Rather than using fixed scanning lines, the system modifies scan paths in real-time based on the rotating substrate's position, ensuring that imaging fields consistently capture the intended regions with correct orientation. This maintains both complete surface coverage and accurate spatial orientation throughout the scanning process.
Solution Approach 2:
The invention uses a composite scanning approach combining multiple scanning patterns and coordinate transformations. The system integrates radial and angular scanning components, applying mathematical transformations to correct for rotation effects. This composite scanning strategy ensures complete surface coverage while maintaining precise orientation accuracy by compensating for rotational distortion through coordinated scanning movements.
Data Source
AI summary
Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.