Optical Alignment Tool for Nucleic Acid Sequencing
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Solution Overview
Problem
There is a need for tools that facilitate accurate calibration and alignment of optical detection systems, particularly in nucleic acid sequencing procedures, where precise alignment and validation of imaging modules are crucial for effective sample detection.
Innovation Solution
An inspection apparatus comprising a translucent or transparent plate with a patterned opaque material and a chamber for light emission, optionally filled with light-emitting fluid, is used to align and validate imaging modules. This apparatus includes fiducial elements and a channel configuration suitable for use in flow cell cartridges, enabling optical alignment and validation of imaging modules in nucleic acid sequencing systems.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional alignment tools are used for optical detection systems, then the alignment process is simpler, but the alignment precision and measurement accuracy are insufficient for high-resolution imaging requirements
Solution Approach 1:
The patent creates a simplified optical copy or representation of the complex alignment target structure through the translucent plate with patterned opaque material. This allows the imaging module to detect and align with fiducial markers that replicate the essential geometric features needed for precise alignment without requiring the full complexity of the actual sample structure
Solution Approach 2:
The patent transforms the alignment target into different optical states by using translucent/transparent regions that allow light transmission and opaque regions that block light. This creates high-contrast optical parameters (light/dark patterns) that are easily detectable by the imaging module, enabling precise measurement of position and orientation parameters for alignment
2Manufacturing precision
If high-resolution imaging capabilities are implemented for accurate alignment, then the alignment accuracy is improved, but the complexity of the inspection apparatus increases
Solution Approach 1:
The patent applies different optical properties to different regions of the plate: translucent/transparent regions for light transmission and fiducial marking, opaque regions for light blocking and pattern formation. This local differentiation of material properties allows the single plate to perform multiple functions (alignment reference, fiducial marking, light management) without requiring multiple separate components, thus reducing overall apparatus complexity while maintaining high alignment accuracy
3Measurement precision
If fiducial elements with transparent or translucent portions are created, then the optical detection sensitivity is enhanced, but the manufacturing complexity of the patterned plate increases
Solution Approach 1:
The patent extracts the fiducial marker function from the bulk material by creating localized transparent or translucent portions within the patterned opaque material. These extracted transparent regions serve as distinct fiducial elements that are easily detectable by optical systems, while the surrounding opaque material provides the structural pattern. This separation of functions (opaque material for pattern, transparent regions for fiducials) simplifies the manufacturing approach compared to creating fiducials from entirely different materials
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution provides high-resolution imaging capabilities, allowing for accurate alignment and validation of imaging modules, enhancing the precision and reliability of nucleic acid sequencing processes by ensuring proper optical alignment and detection of samples.
Implementation Method 1
The light emitting material can include one or more fluorescent or luminescent molecules. For example, the fluorescent molecules can be a Rhodamine dye or an Oxazine dye.
Implementation Method 2
The light emitting material can include one or more fluorescent or luminescent molecules.
Data Source
AI summary
Provided is an inspection apparatus including: (a) a translucent or transparent plate having a bottom surface, at least a portion of the bottom surface having an opaque material printed thereon in a pattern having at least one transparent or translucent portion; and (b) a chamber disposed below the bottom surface, whereby light emitted from the chamber or through the chamber can pass through the at least one transparent or translucent portion.