Optical Analysis Reference Cell Correction for Blood Sample Abnormality Detection
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Solution Overview
Problem
Conventional optical sample analysis methods fail to accurately detect abnormalities in sample color or concentration before reaction with a reagent, leading to potential overlooking of abnormalities, especially in blood samples, and incorrect blank correction data due to light scattering and absorption by the reagent.
Innovation Solution
The method involves irradiating a reaction cell with a sample and reagent, a reference cell without reagent, and a base portion to obtain optical characteristics data, allowing for precise analysis of the unreacted sample and correction of reaction data, using a data processor to determine normalcy and correct for dilution and color changes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the sample is diluted before reaction with the reagent, then the optical data is less influenced by sample color, but the color abnormalities are more likely to be overlooked
Solution Approach 1:
The patent performs preliminary optical measurement of the sample before it reacts with the reagent. This preliminary measurement captures the sample's original color characteristics (yellow for high bilirubin, red for hemolysis, milky white for chyle) before dilution and reaction occur, allowing detection of color abnormalities that would otherwise be lost in the subsequent dilution process.
2Ease of manufacture
If the reagent is provided in solid form in the cell, then the reagent surface scatters and reflects light, but the blank correction data becomes incorrect
Solution Approach 1:
The patent extracts the reagent from the optical measurement path by providing it in a recessed portion of the base plate that is positioned outside the light beam's path. The light beam passes through the sample in the through-hole without encountering the reagent, thereby eliminating light scattering and reflection by the reagent while still allowing the reagent to contact and react with the sample.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables accurate detection of abnormalities such as hemolysis and chyle in blood samples and provides precise optical data correction, reducing the likelihood of incorrect analysis results.
Implementation Method 1
The reaction portion of the sample and the reagent is irradiated with light, whereby optical data on the reaction portion such as the amount of light transmitted or reflected are obtained
Implementation Method 2
the surface of the reagent may readily scatter and reflect light
Data Source
AI summary
A method for analyzing a sample includes the step of irradiating a reaction portion of a sample BL and a reagent 40 in a reaction cell 34A of an analysis unit with light to obtain data D0 indicating optical characteristics of this portion. The method further includes the steps of irradiating a reference cell 34B which is not provided with a reagent 40 with light in a state in which the sample BL is supplied to the cell to obtain reference data D1 indicating optical characteristics of this portion, irradiating a base portion 34C of the analysis unit with light to obtain reference data D2 indicating optical characteristics of this portion, and obtaining data D3 indicating optical characteristics of the sample BL before reaction with the reagent 40 based on the reference data D1 and D2.