Optical Bacterial Sensitivity Detection via Light Attenuation
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Solution Overview
Problem
Current methods for testing the sensitivity of bacterial strains to bacteriophages are time-consuming and require extensive laboratory testing, involving multiple viral strains and bacterial concentrations.
Innovation Solution
A process that uses a device with a light source and an image sensor to analyze the sensitivity of bacterial strains to bacteriophages by monitoring light attenuation and texture changes in images acquired over time, allowing for simultaneous testing of multiple bacterial and phage combinations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional laboratory phagogram tests are performed to determine bacterial sensitivity to phages, then accurate sensitivity data is obtained, but the testing time and number of experiments increase significantly
Solution Approach 1:
The patent replaces traditional mechanical/microscopic observation methods with optical measurement methods. Instead of visually observing bacterial lysis through microscopy, the system uses light transmission measurements through the culture medium to detect bacterial density changes, thereby reducing observation time and increasing throughput while maintaining sensitivity detection accuracy
Solution Approach 2:
The patent changes the measurement parameter from direct visual observation to optical transmission intensity. By measuring light transmission through the culture medium, the system indirectly detects bacterial presence and lysis events, enabling automated, high-throughput screening that reduces testing time while maintaining accurate sensitivity determination
2Adaptability or versatility
If multiple viral strains and phage-to-bacteria ratios are tested simultaneously, then comprehensive sensitivity data is obtained, but the number of required tests increases
Solution Approach 1:
The patent merges multiple test conditions into a single integrated assay. By incorporating multiple viral strains and varying phage-to-bacteria ratios into one culture system that can be simultaneously measured optically, the system reduces the total number of separate tests required while maintaining comprehensive sensitivity data across all tested conditions
Solution Approach 2:
The patent creates a universal testing platform that can simultaneously evaluate multiple phage strains, bacterial strains, and phage-to-bacteria ratios using the same optical measurement system. This multi-functional approach eliminates the need for separate specialized assays for each condition, reducing overall device complexity and testing burden
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables early detection of bacterial lysis by phages and significantly reduces the time required to determine bacterial sensitivity, facilitating a more efficient phage therapy approach.
Implementation Method 1
monitoring light attenuation and texture changes in images acquired over time
Implementation Method 2
acquisition by an image sensor of images of the sample, at successive measurement times
Data Source
Figure 1~2A
Figure 2B~2C
Figure 2D~2E
AI summary
A method for determining the sensitivity of a bacterial strain of interest to a viral strain of bacteriophages, the method comprising: a) preparing a sample (20), comprising bringing bacteria (2) belonging to the bacterial strain of interest into contact with bacteriophages (3), each bacteriophage belonging to the same viral strain, the bacteria being either in a liquid medium or in an agar medium; b) arranging the sample between a light source (10) and an image sensor (30), the light source emitting a light wave (11) in an emission spectral band between 500 nm and 600 nm; c) illuminating the sample with the light source and acquiring at least one image of the sample, by the image sensor, in the emission spectral band, no image-forming optics being arranged between the sample and the image sensor;d) from the acquired images, determination of the sensitivity of the bacterial strain of interest to the viral strain.