Optical Cell Analysis Device with Disposable Light-Sensitive Plate

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Solution Overview

Problem

Conventional cell analyzing devices are costly, unable to cultivate cells, and suffer from low detection accuracy due to reliance on expensive sensors and resistance-based detection methods.

Innovation Solution

A cell/particle analyzing device utilizing low-cost light-sensing plates and optical equipment to generate and receive light beams for accurate analysis, while also providing a culture medium for cell cultivation, using a light-emitting unit, light-diverting unit, and receiving units to capture forward and side scattering lights for precise biological characterization.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If expensive sensors are used to receive light beams for cell analysis, then detection accuracy is improved, but device cost increases

Engineering Contradiction:
Improvedetection accuracyVSAvoiddevice cost
Core Design Contradiction:
Measurement precisionVSEase of manufacture

Solution Approach 1:

The patent uses a light-sensitive plate to capture optical images of cells, creating a visual copy of cell morphology and characteristics. This optical copying approach replaces expensive sensors while maintaining detection accuracy through image-based analysis of cell forward scattering light, side scattering light, and fluorescent light signals.

Inventive Principle:
Principle #26Copying

Solution Approach 2:

The invention employs disposable or low-cost light-sensitive plates instead of expensive, maintenance-intensive sensors. These plates can be replaced easily and at low cost, eliminating the high maintenance costs associated with conventional sensors while providing sufficient detection capability for cell analysis.

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

2Adaptability or versatility

If a culture dish with resistance detection is used for cell cultivation and analysis, then cell cultivation capability is added, but device cost increases

Engineering Contradiction:
Improvecell cultivation capabilityVSAvoiddevice cost
Core Design Contradiction:
Adaptability or versatilityVSEase of manufacture

Solution Approach 1:

The patent integrates multiple functions into a single device: the culture dish serves both as a cultivation vessel and as an optical chamber for light beam transmission. The same culture dish that grows cells also serves as the medium through which light passes to reach the light-sensitive plate, eliminating the need for separate expensive resistance detection systems while maintaining both cultivation and analysis capabilities.

Inventive Principle:
Principle #6Universality (Multi-functionality)

3Adaptability or versatility

If resistance-based detection is used for cell analysis, then cell cultivation and analysis are combined, but detection accuracy decreases

Engineering Contradiction:
Improvecombined cultivation and analysisVSAvoiddetection accuracy
Core Design Contradiction:
Adaptability or versatilityVSMeasurement precision

Solution Approach 1:

The patent replaces the electrical resistance detection mechanism with an optical detection system. Instead of measuring electrical resistance changes in the culture dish, the system uses light beams (laser or LED) that pass through the culture dish and cells to the light-sensitive plate, creating optical images that provide accurate morphological and biological characteristic data without compromising detection precision.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The device achieves cost-effective and accurate analysis of cells or particles by using affordable light-sensing plates and optical equipment, enabling real-time analysis during cell culture with improved detection accuracy and reduced maintenance costs.

Implementation Method 1

a light-emitting unit (1) for generating a light beam (101)

Methodology Applied
Scientific EffectLight emission: Light Emitting Diode

Implementation Method 2

the cells 80 are orderly energized by the laser beam 82 to generate a forward scattering light 83, a side scattering light 84 and a fluorescent light 85

Methodology Applied
Scientific EffectLight scattering: Scattering

Implementation Method 3

a light-sensitive plate (4, 41) to receive the light beam (101)

Methodology Applied
Scientific EffectPhotoelectric conversion: Photoelectric Effect

Implementation Method 4

fluorescent dyes and disposed into a sample tube thereafter. The cells 80 are orderly energized by the laser beam 82 to generate a forward scattering light 83, a side scattering light 84 and a fluorescent light 85

Methodology Applied
Scientific EffectFluorescence: Fluorescence

Data Source

PatentUS8699028B2Real-time cell/particle analyzing device with optical detection and cell cultivation functions
Publication Date: 2014.04.15 NAT PINGTUNG UNIV OF SCI & TECH
  • US8699028B2 patent drawing
  • US8699028B2 patent drawing
  • US8699028B2 patent drawing

AI summary

A cell/particle analyzing device includes a light-emitting unit, a light-diverting unit, a first receiving unit and a second receiving unit. The light-emitting unit generates a first light beam. The light-diverting unit is connected to the light-emitting unit and has an input end, a bidirectional transceiving end and an output end. The input end receives the first light beam generated by the light-emitting unit. The bidirectional transceiving end transmits the first light beam generated by the light-emitting unit and receives a second light beam. The output end outputs the second light beam. The first receiving unit is connected to the output end of the light-diverting unit and receives the second light beam. The second receiving unit is aligned with the bidirectional transceiving end.