Optical Detection Verification Using Standard Nucleic Acid Samples
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Solution Overview
Problem
Conventional methods for verifying the optical signal detection and temperature control performance of optical detection apparatuses are time-consuming and unreliable, as they require separate verification processes and can be affected by external thermometer introduction, making accurate temperature control challenging.
Innovation Solution
A method using a standard sample with a known nucleic acid sequence and complementary strand to verify optical signal detection and temperature control performance by measuring optical signal intensity and melting temperature changes, allowing simultaneous evaluation of both performance aspects without external thermometer intervention.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If separate verification processes are used for optical signal detection and temperature control performance, then verification coverage is improved, but verification time and complexity increase
Solution Approach 1:
The patent combines optical signal detection verification and temperature control verification into a single integrated process using a standard sample with known properties. Both verification aspects are performed simultaneously through one measurement sequence, eliminating the need for separate verification processes and reducing total verification time while maintaining comprehensive coverage.
Solution Approach 2:
The standard sample serves multiple functions: it provides a known optical signal response for detection verification and a known melting temperature for temperature control verification. This multi-functional standard sample enables both verification objectives to be achieved through a single test protocol.
2Measurement precision
If external thermometers are introduced for temperature verification, then temperature measurement capability is improved, but measurement accuracy deteriorates due to interference with the sample
Solution Approach 1:
The standard sample itself provides the temperature verification information through its known melting temperature. The sample's inherent thermal properties are used to verify temperature control accuracy without requiring external measurement devices that would interfere with the sample or the optical detection process.
Solution Approach 2:
The standard sample acts as an intermediary that translates temperature control performance into measurable optical signal changes. By monitoring the optical signal response of the standard sample during temperature changes, the system indirectly verifies temperature control accuracy without direct physical interference from external thermometers.
3Reliability
If multiple verification steps are performed sequentially, then verification thoroughness is improved, but productivity decreases
Solution Approach 1:
Multiple verification steps for optical detection and temperature control are merged into a single simultaneous verification process. The standard sample enables both verification objectives to be accomplished in one experimental run, dramatically improving verification efficiency while maintaining thoroughness through the multi-functional design of the standard sample.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables reliable and efficient verification of optical signal detection and temperature control performance, ensuring accurate analysis results without the need for external thermometer introduction, thus improving the reliability and simplicity of performance testing.
Implementation Method 1
measuring the optical signal intensity of the standard sample with the detection unit
Implementation Method 2
determining the melting temperature of the standard sample from a change in the optical signal intensity accompanying a change in the temperature
Data Source
AI summary
A method is provided in which with respect to an optical detection apparatus including an optical detection unit and a temperature control unit, whether optical signal detection and temperature control are performed accurately, i.e. the performance thereof, can be verified simply with high reliability. With respect to an optical detection apparatus including an optical detection unit for detecting an optical signal of a sample and a temperature control unit for controlling temperature of the sample, the optical signal detection performance and temperature control performance are verified by the following method. First, a standard sample containing a nucleic acid sequence and a strand complementary thereto that have a known optical signal intensity and Tm value is provided, the temperature of the standard sample is increased or decreased with the temperature control unit, and optical signal intensity of the standard sample is measured with the detection unit. On the other hand, the melting temperature of the standard sample is determined from a change in the optical signal intensity accompanying a change in the temperature. The measured optical signal intensity and melting temperature of the standard sample are compared to the known optical signal intensity and melting temperature of the standard sample, respectively, and thereby it is verified whether the optical signal detection performance of the detection unit and the temperature control performance of the temperature control unit are accurate.