Gas Analysis Wells With Optical Detection and Controlled Gas Flow
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Solution Overview
Problem
Existing methods for analyzing gas effects on samples, such as blood, are limited by the need for additional detectors, inability to analyze multiple samples simultaneously, and lack of control over gas composition, leading to inefficient and time-consuming processes.
Innovation Solution
A device and process using a housing with gas conduits connected to sample wells, allowing continuous delivery of gas with predetermined concentrations, and a spectrophotometer for optical measurement without additional detectors, enabling simultaneous analysis of multiple samples in parallel.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If multiple detectors are used to analyze gas concentration and absorption/desorption, then measurement accuracy is improved, but device complexity increases
Solution Approach 1:
The patent employs a single spectrophotometer that performs multiple measurement functions: measuring gas concentration, absorption, and desorption capacity by analyzing optical property changes of gas-sensitive analytes under different gas conditions, replacing the need for multiple specialized detectors
Solution Approach 2:
The system measures gas effects by detecting changes in optical parameters (absorbance, fluorescence, luminescence) of gas-sensitive analytes in response to varying gas concentrations and compositions, converting gas concentration measurements into optical signal measurements
2Productivity
If samples are analyzed sequentially, then equipment simplicity is maintained, but analysis time increases
Solution Approach 1:
The patent divides the sample set into multiple aliquots distributed across an array of sample wells in a microtiter plate, allowing parallel processing of multiple samples simultaneously while using a single spectrophotometer
Solution Approach 2:
The system transitions from sequential one-dimensional sample analysis to parallel multi-dimensional analysis by arranging samples in a spatial array of wells, enabling simultaneous exposure to different gas compositions and concurrent optical measurements
3Reliability
If gas composition is not controlled, then equipment simplicity is maintained, but measurement reliability decreases
Solution Approach 1:
The patent implements a gas source system that pre-prepares and delivers gas compositions with predetermined and controlled concentrations of gas components to each sample well before measurements begin, ensuring reliable and reproducible measurement conditions
Solution Approach 2:
The system uses a gas conduit system as an intermediary to distribute controlled gas compositions from the gas source to multiple sample wells, mediating between the gas source and samples to ensure precise gas delivery and composition control
4Stability of the object's composition
If buffer is added to samples, then measurement stability is improved, but sample integrity changes
Solution Approach 1:
The patent measures gas effects on gas-sensitive analytes directly in the original sample without adding buffers or other reagents, allowing the sample to maintain its natural composition and integrity while still providing stable and reproducible measurements
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Facilitates rapid and efficient analysis of gas concentration, absorption, and desorption effects on samples without additional detectors, allowing for precise measurement of gas effects on samples like blood, with minimal sample volume and no buffer addition.
Implementation Method 1
measuring the change in an optical property of the gas-sensitive analyte in response to a gas concentration
Implementation Method 2
a gas conduit for delivering a gas flow from a gas source through a sample well
Data Source
AI summary
The invention provides a device and a process for analysing the effect of a concentration of a gas on a sample, especially measurement of the gas concentration within a sample, and/or measurement of the effect of the absorption and/or desorption of a gas by the sample, using spectrophotometric detection of a gas-sensitive and optically detectable analyte of the sample while measuring the concentration of the gas in a gas composition that is continuously delivered to each sample well in order to contact the samples with a gas having a predetermined and/or measured concentration, respectively a pre-determined and/or measured partial pressure.