Optical Measurement Method Using Sensitivity Parameter Slope
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Solution Overview
Problem
Existing optical measurement techniques in laboratory settings face challenges in accurately comparing light intensities measured at different sensitivity parameters, leading to reduced accuracy when detecting multiple analytes with varying concentrations, due to limitations in detector device ranges and the need for precise calibration.
Innovation Solution
A method utilizing multiple sensitivity parameters to determine reference points and calculate analyte values based on the slope of lines defined by these points, allowing for accurate comparison of intensities across different sensitivity settings, with the use of optical standard samples to establish standardization points for improved accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If multiple sensitivity parameters are used to expand the detectable intensity range, then the range of detectable light intensities is increased, but the accuracy of comparing intensities measured at different sensitivity parameters is reduced
Solution Approach 1:
The patent changes the sensitivity parameter of the detector device to adapt to different light intensity ranges. By systematically varying the sensitivity parameter and establishing reference points at each level, the system can measure both weak and strong fluorescence signals accurately. The key is that reference measurements are taken at the same sensitivity parameter as the sample measurements, enabling accurate comparison through ratio calculation.
Solution Approach 2:
The patent introduces reference light sources with known intensities as intermediaries to bridge measurements taken at different sensitivity parameters. These reference measurements serve as calibration points that allow the system to translate measurements across different sensitivity levels. The ratio of sample intensity to reference intensity eliminates the need for absolute intensity calibration, resolving the accuracy problem.
2Measurement precision
If a single sensitivity parameter is used to maintain measurement accuracy, then the accuracy of intensity comparison is improved, but the range of detectable light intensities is limited
Solution Approach 1:
The patent segments the measurement process into multiple sensitivity parameter levels. Instead of using a single sensitivity setting, the system divides the measurement range into segments, each measured at an appropriate sensitivity level. Reference points are established at each segment boundary, allowing the system to maintain high accuracy within each segment while covering a broad overall intensity range.
Solution Approach 2:
The patent makes the sensitivity parameter dynamic rather than fixed. The system automatically adjusts the sensitivity parameter based on the expected or measured light intensity, selecting the optimal sensitivity level for each measurement. This dynamic adaptation allows the system to maintain high measurement accuracy across varying intensity conditions without being constrained to a single sensitivity setting.
3Adaptability or versatility
If the sensitivity parameter is varied to measure different intensity ranges, then the adaptability to different analyte concentrations is improved, but the calibration complexity of the laboratory apparatus increases
Solution Approach 1:
The patent implements self-calibration through automatic reference measurements. The system performs reference measurements at each sensitivity parameter level using built-in reference light sources, eliminating the need for manual calibration by the user. The control device automatically processes the reference data and applies the appropriate calibration factors, reducing the complexity of the calibration process from the user's perspective while maintaining measurement accuracy across multiple sensitivity levels.
Solution Approach 2:
The patent uses feedback from reference measurements to automatically adjust and validate the calibration state. After each sensitivity parameter change, the system performs reference measurements and uses the results to verify proper calibration. This feedback mechanism ensures that the system remains accurately calibrated across different sensitivity levels without requiring complex manual intervention, as the system self-corrects based on the reference data.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach increases the range of detectable light intensities and enhances the accuracy of comparing concentrations of multiple analytes by using a single standardization point for multiple analytes, improving the overall precision of measurements across different sensitivity parameters.
Implementation Method 1
The analyte is marked by a fluorescence marker, which can be excited by the excitation light of a light source. The fluorescence marker receives the light and emits a sample light, typically at a different wavelength (fluorescence).
Data Source
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Figure 3a~3b
AI summary
The invention is related to a method for the quantitative optical measurement of a characteristic property of at least one analyte in at least one laboratory sample, in particular for the fluorescence measurement of at least one biochemical or biological sample, the method using a laboratory apparatus, which has at least one light source and at least one detector device, the apparatus utilizing at least sensitivity parameter S, which controls the capability of the laboratory apparatus to detect a signal by means of the at lest one detector device, the method using source light for causing the at least one sample to emit a sample light, and the at least one detector device for detecting sample light and utilizing the at least one sensitivity parameter S to detect the corresponding at least one intensity I of the sample light, the method comprising the steps:- determining at least one reference point (S_ref; I_ref); - using at least one first sensitivity parameter S_m1, which is not the same as S_ref, for measuring at least one first intensity I_m1 of sample light as-signed to a first analyte; -determining a quantity Q1, which is a measure for the slope of a line, which is determined by utilizing the at least one reference point (S_ref; I_ref) and the at least one measurement point (S_m1; I_m1); using the quantity Q1 for calculating a first analyte value C_m1, which is dependent on Q1 and which is characteristic for a property of the first analyte, in particular for a concentration of the first analyte in the at least one sample, in particular according to the formula Q1 = (I_m1 - I_ref) / (S_m1 - S_ref). The method, further, is related to a laboratory apparatus, which is configured to apply the method according to the invention.