Optical Probe for Multiwell Plate Fluorescence and Light Scattering
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Solution Overview
Problem
Current systems for measuring fluorescence in multiwell plates face challenges in maximizing signal-to-noise ratio while minimizing stray light, limiting high-throughput analysis capabilities.
Innovation Solution
A simplified optical system using a single probe with a bundle of fibers for both excitation and emission, positioned close to the multiwell plate with a beveled barrel to reduce back reflections, and optionally combined with a separate probe for light scattering measurements, eliminating additional optics and interfaces to minimize stray light.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a conventional optical system with multiple optics and interfaces is used for fluorescence measurement, then the system can perform measurements, but stray light increases and signal-to-noise ratio decreases
Solution Approach 1:
The patent removes unnecessary optical interfaces and components from the measurement path. By using a simplified optical system with direct fiber-to-plate coupling and eliminating intermediate optics, the number of interfaces that generate stray light is reduced, thereby improving signal-to-noise ratio while reducing system complexity
Solution Approach 2:
The patent addresses back-reflection issues by using a beveled barrel design that directs reflected light away from the detection path. This converts the potentially harmful back-reflection into a beneficial arrangement where reflections are redirected to safe zones, improving measurement precision without requiring complex additional optics
2Adaptability or versatility
If multiple separate probes are used for fluorescence and light scattering measurements, then comprehensive measurements can be performed, but alignment complexity and cost increase
Solution Approach 1:
The patent combines fluorescence and light scattering measurement capabilities into a single integrated probe assembly. The probe contains both fluorescence detection fibers and light scattering detection fibers, allowing both measurements to be performed simultaneously or sequentially from the same position, thereby reducing alignment complexity while maintaining comprehensive measurement capability
Solution Approach 2:
The single probe design serves multiple functions by incorporating both fluorescence detection and light scattering detection capabilities. This universal probe can perform different types of measurements without requiring separate specialized probes, reducing both alignment complexity and overall system cost
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This configuration enhances the signal-to-noise ratio, reduces alignment and cost, and enables high-throughput analysis of fluorescence and light scattering data from multiwell plates with reduced noise and increased efficiency.
Implementation Method 1
A fiber optic apparatus is provided for measuring fluorescence and light scattering from a liquid sample contained within a well of a multiwell plate. The apparatus includes an optical probe in the form of a bundle of optical fibers with one fiber acting as an illumination vehicle and a second fiber acting as a collection vehicle.
Implementation Method 2
The sample is illuminated with light at a first wavelength which causes the illuminated portion of the sample to fluoresce at a second, different wavelength.
Implementation Method 3
a second fiber acting as a collection vehicle for scattered and unscattered light
Data Source
AI summary
An apparatus for the high throughput measurement of optical properties of liquid samples placed into the wells of a multiwell plate is disclosed. An optical fiber within a fiber bundle containing no corrective optics between the fiber ends and the well plate bottom illuminates the sample in order to induce fluorescence, and multiple fibers collect emission radiation and transmit it to a fluorescence detector such as a spectrometer. Other embodiments involve a light scattering illumination source with detection fibers located in either the same bundle containing the fluorescence monitoring fibers or an independent light scattering detection bundle for the measurement of static and/or dynamic light scattering. Some embodiments of the invention permit the measurement of phase analysis light scattering. Thus the measurement of multiple optical properties of a liquid sample may be made simultaneously or in succession. A method for these measurements is also disclosed.