Optical Probe Autofocus Using Wavelength Shift Defocus Detection

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Solution Overview

Problem

Existing autofocus systems in optical microscopes struggle to accurately detect and correct defocus, particularly when imaging samples with uneven or non-flat surfaces, leading to suboptimal imaging results.

Innovation Solution

A passive autofocus system that utilizes a wavelength-sensitive photodetector to capture sub-images formed by light in different wavelength ranges, such as red and blue, to determine the degree of defocus based on the lateral shift between these sub-images, allowing for precise focus adjustment without additional energy sources.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If a passive autofocus system using wavelength-sensitive photodetector and lateral shift measurement is implemented, then measurement precision of defocus is improved, but device complexity increases due to additional optics and multi-wavelength light source requirements

Engineering Contradiction:
Improvedefocus detection accuracyVSAvoidoptical system complexity
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The patent segments the detected image into multiple sub-images based on wavelength ranges. The photodetector array captures light across different wavelengths, and the computing device separates these into distinct sub-images (e.g., blue, green, red channels). This segmentation enables lateral shift measurement between wavelength-specific sub-images to determine defocus, achieving high measurement precision while using standard photodetector technology.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent makes the imaging system multi-functional by enabling it to simultaneously perform true-color imaging and autofocus detection using the same optical path and photodetector. The system processes the same captured image to generate both color information (through wavelength-based sub-image separation) and focus information (through lateral shift measurement), eliminating the need for separate autofocus hardware and reducing overall device complexity.

Inventive Principle:
Principle #6Universality (Multi-functionality)

2Measurement precision

If multiple light beams of different wavelength ranges are used for defocus detection, then measurement precision is improved, but loss of energy increases due to multiple light sources or filtering requirements

Engineering Contradiction:
Improvedefocus measurement accuracyVSAvoidlight energy loss
Core Design Contradiction:
Measurement precisionVSLoss of energy

Solution Approach 1:

The patent merges multiple wavelength ranges into a single imaging process. Instead of using separate light sources or sequential filtering that would cause energy loss, the system uses a broadband light source that illuminates the sample with all visible wavelengths simultaneously. The photodetector array captures the combined reflected light, and computational methods separate the wavelengths to generate sub-images for lateral shift analysis, preserving light energy throughout the process.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The system uses the light reflected from the sample for dual purposes: both forming the true-color image and providing the information needed for defocus detection. The same reflected light that carries color information also contains the lateral shift information needed for autofocus, eliminating the need for additional illumination energy and achieving self-service in terms of light utilization.

Inventive Principle:
Principle #25Self-service

3Measurement precision

If lateral shift between sub-images is used to determine defocus, then measurement precision is improved, but difficulty of detecting and measuring increases due to image processing complexity

Engineering Contradiction:
Improvedefocus detection precisionVSAvoidimage processing complexity
Core Design Contradiction:
Measurement precisionVSDifficulty of detecting and measuring

Solution Approach 1:

The system implements a feedback mechanism where the computing device continuously calculates the lateral shift between corresponding features in different wavelength sub-images and uses this information to determine the defocus state. This feedback loop enables real-time autofocus adjustment, with the system monitoring and responding to focus changes based on precise lateral shift measurements, achieving high measurement precision through systematic image processing.

Inventive Principle:
Principle #23Feedback

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables simultaneous autofocusing and true-color imaging by accurately determining the defocus through the lateral shift of sub-images, improving imaging quality on varied sample surfaces without interfering with the normal use of the spectroscopy system.

Implementation Method 1

a wavelength-sensitive photodetector... the first sub-image being formed with light within the first wavelength range detected by the wavelength-sensitive photodetector, the second sub-image being formed with light within the second wavelength range detected by the wavelength-sensitive photodetector

Methodology Applied
Scientific EffectPhotoelectric effect: Photoelectric Effect

Implementation Method 2

optics configured to illuminate a portion of a sample with the first and second light beams from different directions of incidence and project an image of at least a part of the illuminated portion of the sample onto the wavelength-sensitive photodetector

Methodology Applied
Scientific EffectRefraction: Refraction

Data Source

PatentUS20260043993A1Method and system for detecting defocus of optical system
Publication Date: 2026.02.12 THERMO ELECTRONICS SCI INSTR LLC
  • US20260043993A1 patent drawing
  • US20260043993A1 patent drawing
  • US20260043993A1 patent drawing

AI summary

In one example, a method for detecting defocus of an optical probe includes obtaining a first sub-image and a second sub-image of an image projected by the optics of the corresponding optical system onto a wavelength-sensitive photodetector in response to a sample region being illuminated with a first light beam of a first wavelength range and a second light beam of a second wavelength range. The first sub-image is formed with light detected by the wavelength-sensitive pixelated photodetector within the first wavelength range. The second sub-image is formed with light detected by the wavelength-sensitive pixelated photodetector within the second wavelength range. The method further includes determining a degree of defocus based on the first sub-image and the second sub-image.