Optical Readout Imaging System for Western Blot Signal Control
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Solution Overview
Problem
Current Western Blot technology faces issues with image errors and signal distortion due to manually controlled mechanisms and the inability to adjust voltage and current, making it difficult to apply voltage locally during the shifting process.
Innovation Solution
An optical readout imaging system comprising a first electrode, a thin film, a biomolecule transfer layer, and a second electrode, where the second electrode is driven to transfer specimens from one position to another within the biomolecule transfer layer, and then to the thin film, allowing for precise control of voltage application to avoid signal distortion.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If manually controlled mechanisms are used in Western Blot, then the operation flexibility is maintained, but image errors and signal distortion occur due to inability to adjust voltage and current locally
Solution Approach 1:
The electrode is divided into multiple independently controllable sub-electrodes arranged in a grid pattern. Each sub-electrode can be controlled separately to apply voltage and current at specific locations, enabling precise local control during the shifting process and eliminating manual operation limitations.
Solution Approach 2:
The manual control mechanism is replaced with an automated electrode control system that uses electrical signals to precisely control voltage and current application. This substitution eliminates the limitations of manual operation and enables automated, precise control throughout the Western Blot process.
2Reliability
If voltage and current are not adjustable locally, then the device structure remains simple, but signal distortion occurs during the shifting process
Solution Approach 1:
The electrode is segmented into multiple sub-electrodes that can be independently controlled. This segmentation allows voltage and current to be applied locally at specific positions during the shifting process, preventing signal distortion while maintaining a manageable structural complexity through modular design.
Solution Approach 2:
The electrode control system transitions from static, uniform voltage application to dynamic, variable voltage application. The system can adjust voltage and current in real-time at different locations during the shifting process, enabling precise control to maintain signal accuracy throughout the experiment.
3Measurement precision
If manual control is used, then the operation is flexible, but quantification of trace targeted protein becomes inaccurate
Solution Approach 1:
Manual operation is replaced with an automated electrode control system that precisely controls voltage and current application. This automation ensures consistent and accurate protein quantification by eliminating human error in timing and positioning, while the system maintains ease of use through automated protocols.
Solution Approach 2:
The system incorporates feedback mechanisms to monitor and adjust voltage and current application in real-time during the shifting process. This feedback control ensures accurate protein quantification by automatically optimizing conditions, while the automated nature of the system maintains operational simplicity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This solution enables accurate and precise biochemical detection by minimizing signal distortion and image errors, allowing for the effective quantification of proteins through controlled voltage application, improving the reliability of Western Blot technology.
Implementation Method 1
driving the second electrode to transfer the specimen from a first position to a second position in the biomolecule transfer layer
Implementation Method 2
driving the second electrode and the first electrode to shift the specimen from the biomolecule transfer layer to the thin film
Data Source
AI summary
The present disclosure provides an optical readout imaging system may include first electrode, a thin film disposed on the first electrode, a biomolecule transfer layer disposed on the thin film, and a second electrode disposed on the biomolecule transfer layer. The present disclosure also provides a biochemical detection method using the optical readout imaging system.