Ammonia-oxidizing <i>Nitrosomonas eutropha </i>strain D23

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Solution Overview

Problem

There is a need for improved beneficial bacteria that can effectively suppress the growth of pathogenic bacteria, particularly those associated with skin disorders and low nitrite levels, with enhanced capabilities in ammonia oxidation and resistance to ammonium ions.

Innovation Solution

An optimized strain of Nitrosomonas eutropha (N. eutropha) designated D23, which exhibits improved growth rates, ammonia oxidation rates, and resistance to ammonium ions, capable of producing nitric oxide and nitrite precursors, is developed and purified for use in treating diseases and conditions related to pathogenic bacteria and skin disorders.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If conventional bacteria are used, then pathogenic bacteria suppression is achieved, but growth rate and ammonia oxidation rate are insufficient

Engineering Contradiction:

Improveammonia oxidation rateVSAvoidpathogenic bacteria suppression efficacy

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent applies parameter changes by optimizing specific physiological parameters of Nitrosomonas eutropha, including growth rate, ammonia oxidation rate, and resistance to ammonium ions. The optimized strain exhibits enhanced metabolic activity and bacterial suppression capability through modified physiological characteristics rather than structural changes.

Inventive Principle:

Principle #35Parameter changes

2Quantity of substance

If ammonia oxidation rate is increased, then nitrite production is enhanced, but resistance to ammonium ions may be compromised

Engineering Contradiction:

Improvenitrite productionVSAvoidresistance to ammonium ions

Core Design Contradiction:

Quantity of substanceVSStability of the object's composition

Solution Approach 1:

The patent simultaneously optimizes multiple parameters including ammonia oxidation rate and ammonium ion resistance through physiological adaptation of the Nitrosomonas eutropha strain. The optimized strain achieves enhanced nitrite production while maintaining stability against ammonium ion toxicity through coordinated physiological changes.

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The optimized N. eutropha strain effectively suppresses pathogenic bacteria growth, enhances nitrite production, and demonstrates improved resistance to ammonium ions, providing a therapeutic benefit for conditions associated with low nitrite levels and skin disorders.

Implementation Method 1

Ammonia oxidizing bacterial of the genus Nitrosomonas are ubiquitous Gram-negative obligate chemolithoautotrophic bacteria with a unique capacity to generate energy exclusively from the conversion of ammonia to nitrite

Methodology Applied

Scientific EffectChemolithoautotrophy:

Implementation Method 2

the conversion of ammonia to nitrite

Methodology Applied

Scientific EffectAmmonia oxidation: Oxidation

Data Source

PatentUS12091652B2Ammonia-oxidizing <i>Nitrosomonas eutropha </i>strain D23

Publication Date: 2024.09.17 AOBIOME LLC

AI summary

This disclosure provides, inter alia, an optimized strain of Nitrosomonas eutropha (N. eutropha) designated D23, D23-100, or AOB D23-100. N. eutropha bacteria disclosed in this application have desirable properties, e.g., optimized properties, such as the ability to suppress growth of pathogenic bacteria, and an enhanced ability to produce nitric oxide and nitric oxide precursors. The N. eutropha herein may be used, for instance, to treat diseases associated with low nitrite levels, skin diseases, and diseases caused by pathogenic bacteria.