Orange EV Hydrogel Composition for Regenerative Wound Healing
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Solution Overview
Problem
Current hydrogels are ineffective in promoting healing processes such as cellular proliferation, cellular migration, or angiogenesis, limiting their therapeutic efficacy in treating skin wounds and lesions.
Innovation Solution
A pharmaceutical composition comprising orange-derived extracellular vesicles (EVs) encapsulated in a hydrogel matrix, which promotes angiogenesis and tissue regeneration by dispersing the EVs in a controlled manner.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If hydrogels are used as wound dressings to maintain moist environment and regulate fluid exchange, then wound bed hydration is improved, but therapeutic efficacy in promoting cellular proliferation, migration and angiogenesis deteriorates
Solution Approach 1:
The invention combines hydrogel with plant-derived extracellular vesicles (EVs) to create a composite material that integrates the moisture-regulating properties of hydrogel with the regenerative capabilities of EVs. The hydrogel serves as a carrier matrix that maintains wound bed hydration while simultaneously delivering EVs that promote cellular proliferation, migration and angiogenesis, thus resolving the contradiction between maintaining stable hydration and achieving reliable therapeutic effects.
Solution Approach 2:
The invention merges two distinct therapeutic approaches: hydrogel-based wound dressing that provides physical protection and moisture regulation, and EV-based therapy that delivers bioactive molecules for tissue regeneration. By combining these two elements into a single formulation, the invention achieves both the hydration stability of hydrogels and the regenerative efficacy of EVs, eliminating the limitation of hydrogels alone.
2Ease of operation
If conventional hydrogels are applied to promote wound healing, then wound bed moisture regulation is improved, but cellular regeneration processes deteriorate
Solution Approach 1:
The invention introduces plant-derived EVs as intermediary carriers that bridge the gap between the hydrogel matrix and the wound tissue. These EVs act as mediators that transfer regenerative signals from the hydrogel formulation to the wound bed, promoting cellular proliferation, migration and angiogenesis while the hydrogel continues to provide moisture regulation. This intermediary mechanism enables the hydrogel to exert both moisture control and regenerative effects.
Solution Approach 2:
The invention changes the functional parameters of the hydrogel by incorporating EVs with specific regenerative properties. The EVs introduce new biological parameters (proliferation-promoting, migration-promoting, angiogenesis-promoting activities) to the hydrogel system, transforming it from a passive moisture-regulating dressing to an active regenerative therapy that simultaneously maintains optimal moisture levels and accelerates cellular regeneration.
Data Source
Figure 1A~1E
Figure 2A~2D
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AI summary
The invention relates to a pharmaceutical composition in a hydrogel form, comprising orange-derived extracellular vesicles (EVs) having a diameter ranging from 10 to 500 nm and showing pro-angiogenic activity, for use in a tissue repair and/or regenerative therapeutic method. In the pharmaceutical composition of the invention, the orange-derived EVs are dispersed in the hydrogel matrix and releasable from said matrix. The invention also relates to a method for the preparation of said pharmaceutical composition.