Orbital Shaker for Mesenchymal Stem Cell Activation

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Solution Overview

Problem

Existing methods for promoting anti-inflammatory and regenerative properties in tissues containing mesenchymal stem cells are not sufficiently safe, practical, or effective in avoiding contamination and ensuring reliable, cost-effective results.

Innovation Solution

An apparatus and method involving mechanical activation of lipoaspirated adipose tissue using an orbital shaker to enhance the anti-inflammatory and regenerative capabilities of mesenchymal stem cells, while ensuring safety, practicality, and minimizing contamination.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If mechanical activation is applied to adipose tissue to enhance anti-inflammatory properties, then regenerative capability is improved, but risk of contamination increases

Engineering Contradiction:
Improveregenerative capabilityVSAvoidcontamination risk
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The system divides the adipose tissue processing into separate sterile compartments: a first container for mechanical activation and a second container for sterile transfer. This segmentation prevents contamination by physically isolating the mechanical activation process from the sterile tissue handling process.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

A sterile transfer device acts as an intermediary between the non-sterile mechanical activation container and the sterile transplantation container. This intermediary component allows material transfer while maintaining sterile barriers, preventing contamination during the transfer process.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Reliability

If complex mechanical activation devices are used to enhance stem cell properties, then treatment effectiveness improves, but device complexity and cost increase

Engineering Contradiction:
Improvetreatment effectivenessVSAvoidapparatus complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The mechanical activation device is designed to be multifunctional, serving both the mechanical activation function and the sterile containment function. The single-use container system integrates multiple functions (activation, containment, transfer preparation) into one device, reducing overall system complexity while maintaining effectiveness.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Solution Approach 2:

The system employs single-use disposable containers for mechanical activation and sterile transfer. This approach eliminates the need for complex sterilization and cleaning systems, reducing device complexity and cost while ensuring sterility and reliability for each use.

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

3Ease of operation

If manual handling procedures are used for tissue transfer, then operational simplicity is maintained, but contamination risk and inconsistency increase

Engineering Contradiction:
Improveoperational simplicityVSAvoidconsistency and sterility
Core Design Contradiction:
Ease of operationVSReliability

Solution Approach 1:

The sterile transfer device is designed to be self-contained and self-sterilizing through its sealed container design. The system prepares its own sterile environment without requiring external sterilization procedures, maintaining both operational simplicity and consistent sterility.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The system performs preliminary sterilization and preparation actions during the manufacturing of single-use containers. Sterile barriers are pre-established in the container design, eliminating the need for complex manual sterilization procedures during operation while ensuring consistent sterility.

Inventive Principle:
Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method effectively promotes anti-inflammatory and regenerative properties in tissues, leading to improved tissue repair and functional recovery in both animal and human subjects, with enhanced safety, practicality, and cost-effectiveness.

Implementation Method 1

The invention also relates to a method for promoting anti-inflammatory properties and for improving the repairing capability of a tissue containing mesenchymal stem cells, characterized in that it comprises a step of mechanical activation performed by means of the application of an orbital and/or eccentric shaking force to the adipose tissue

Methodology Applied
Scientific EffectMechanical vibration: Vibration

Data Source

PatentUS20250136945A1Tissue containing mesenchymal stem cells and apparatus and method for promoting the Anti-inflammatory, antinociceptive properties and for improving the repairing capability of a tissue containing mesenchymal stem cells
Publication Date: 2025.05.01 SURGERE SRL
  • US20250136945A1 patent drawing
  • US20250136945A1 patent drawing
  • US20250136945A1 patent drawing

AI summary

Provided is an apparatus for promoting anti-inflammatory properties and the repairing capability of a tissue containing mesenchymal stem cells. The apparatus has a containment and support device for washed lipoaspirated adipose tissue containing mesenchymal stem cells. The containment and support device has an external containment body which forms an access mouth closable by a closure lid, a transfer body being accommodatable within the external containment body and forming at least one locking seat for a respective syringe-like body which accommodates the washed lipoaspirated adipose tissue. A mechanical activation device has an orbital shaker which forms means for accommodating the containment and support device.