Orbital Shaker for Mesenchymal Stem Cell Activation
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Solution Overview
Problem
Existing methods for promoting anti-inflammatory and regenerative properties in tissues containing mesenchymal stem cells are not sufficiently safe, practical, or effective in avoiding contamination and ensuring reliable, cost-effective results.
Innovation Solution
An apparatus and method involving mechanical activation of lipoaspirated adipose tissue using an orbital shaker to enhance the anti-inflammatory and regenerative capabilities of mesenchymal stem cells, while ensuring safety, practicality, and minimizing contamination.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If mechanical activation is applied to adipose tissue to enhance anti-inflammatory properties, then regenerative capability is improved, but risk of contamination increases
Solution Approach 1:
The system divides the adipose tissue processing into separate sterile compartments: a first container for mechanical activation and a second container for sterile transfer. This segmentation prevents contamination by physically isolating the mechanical activation process from the sterile tissue handling process.
Solution Approach 2:
A sterile transfer device acts as an intermediary between the non-sterile mechanical activation container and the sterile transplantation container. This intermediary component allows material transfer while maintaining sterile barriers, preventing contamination during the transfer process.
2Reliability
If complex mechanical activation devices are used to enhance stem cell properties, then treatment effectiveness improves, but device complexity and cost increase
Solution Approach 1:
The mechanical activation device is designed to be multifunctional, serving both the mechanical activation function and the sterile containment function. The single-use container system integrates multiple functions (activation, containment, transfer preparation) into one device, reducing overall system complexity while maintaining effectiveness.
Solution Approach 2:
The system employs single-use disposable containers for mechanical activation and sterile transfer. This approach eliminates the need for complex sterilization and cleaning systems, reducing device complexity and cost while ensuring sterility and reliability for each use.
3Ease of operation
If manual handling procedures are used for tissue transfer, then operational simplicity is maintained, but contamination risk and inconsistency increase
Solution Approach 1:
The sterile transfer device is designed to be self-contained and self-sterilizing through its sealed container design. The system prepares its own sterile environment without requiring external sterilization procedures, maintaining both operational simplicity and consistent sterility.
Solution Approach 2:
The system performs preliminary sterilization and preparation actions during the manufacturing of single-use containers. Sterile barriers are pre-established in the container design, eliminating the need for complex manual sterilization procedures during operation while ensuring consistent sterility.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively promotes anti-inflammatory and regenerative properties in tissues, leading to improved tissue repair and functional recovery in both animal and human subjects, with enhanced safety, practicality, and cost-effectiveness.
Implementation Method 1
The invention also relates to a method for promoting anti-inflammatory properties and for improving the repairing capability of a tissue containing mesenchymal stem cells, characterized in that it comprises a step of mechanical activation performed by means of the application of an orbital and/or eccentric shaking force to the adipose tissue
Data Source
AI summary
Provided is an apparatus for promoting anti-inflammatory properties and the repairing capability of a tissue containing mesenchymal stem cells. The apparatus has a containment and support device for washed lipoaspirated adipose tissue containing mesenchymal stem cells. The containment and support device has an external containment body which forms an access mouth closable by a closure lid, a transfer body being accommodatable within the external containment body and forming at least one locking seat for a respective syringe-like body which accommodates the washed lipoaspirated adipose tissue. A mechanical activation device has an orbital shaker which forms means for accommodating the containment and support device.


