Organ Transplantability Evaluation via Perfusion Marker Analysis
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Solution Overview
Problem
Conventional organ transplantation methods lack objective criteria for determining the transplantability of organs, leading to potential primary non-function (PNF) issues, especially with vital organs like the heart, lung, and liver, resulting in graft loss and patient risk.
Innovation Solution
A method involving perfusion of organs with a perfusate to evaluate function by measuring changes in marker substances over time using mass spectrometry, providing an objective indicator for transplantability through a set evaluation criterion.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional transplantation methods relying on surgeon experience and prompt histopathological diagnosis are used, then the decision-making process is simple and quick, but the accuracy of determining organ transplantability is insufficient leading to primary non-function
Solution Approach 1:
The invention performs preliminary evaluation of organ function before transplantation by measuring marker substance contents and calculating indicators. This advance assessment allows determination of organ transplantability prior to the actual transplantation procedure, preventing primary non-function while maintaining a manageable evaluation process through standardized measurements and calculations.
2Quantity of substance
If organs from cardiac arrest donors are used to address organ shortage, then the supply of donor organs increases, but the risk of primary non-function and graft loss increases due to lack of objective evaluation criteria
Solution Approach 1:
The invention establishes a feedback mechanism by measuring marker substance contents at different time points during perfusion, calculating indicators that reflect organ function status, and using these indicators to determine transplantability. This continuous monitoring and assessment provides reliable feedback on organ viability, enabling safe use of cardiac arrest donor organs while maintaining high transplant success rates.
3Measurement precision
If objective evaluation criteria are introduced to prevent primary non-function, then the accuracy of transplantability determination improves, but the evaluation process and time required increase
Solution Approach 1:
The invention measures marker substance contents at specific key time points during perfusion rather than continuous monitoring, and calculates indicators based on selected critical parameters. This partial measurement approach provides sufficient objective evaluation data to determine transplantability while minimizing the time required for the assessment process.
Data Source
AI summary
A method of evaluating a function of an organ for transplantation harvested from a living body, the method includes preparing data representing change over time in content of marker substances in accordance with a perfusion, taking a first tissue sample from the organ for transplantation at first timing in a reperfusion of the organ for transplantation, taking a second tissue sample from the organ for transplantation at second timing after the first timing in the reperfusion, measuring contents of the marker substances in the first tissue sample, measuring contents of the marker substances in the second tissue sample, calculating change in content at the second timing as compared with the first timing for each of the marker substances, and calculating an indicator relating to evaluation of the function of the organ for transplantation using the calculated change in content and the data.