Integrated Organ Printing System for Stable Tissue Constructs

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Solution Overview

Problem

Conventional organ printing technologies face challenges in maintaining the pre-defined shape of tissue-engineered scaffolds due to the low mechanical stability of low viscosity materials, which hinders the preservation of complex structures and the protection of immature cells.

Innovation Solution

An integrated organ printing system with a support stage, temperature regulation, and a CAD/CAM system for precise deposition of structural support polymers and cell-containing compositions in multiple layers, allowing for the creation of high-strength three-dimensional constructs with accurate cell localization.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If low viscosity gel materials are used for ink-jet cell printing, then cell viability and printing accuracy are improved, but mechanical stability and structural integrity deteriorate

Engineering Contradiction:
Improveprinting accuracyVSAvoidmechanical stability
Core Design Contradiction:
Measurement precisionVSStrength

Solution Approach 1:

The patent uses composite materials by combining low viscosity gel materials (for cell viability and printing accuracy) with high strength scaffold materials (for mechanical stability). The scaffold provides structural support while the gel contains viable cells, creating a composite tissue construct that achieves both printing precision and mechanical integrity.

Inventive Principle:
Principle #40Composite materials

Solution Approach 2:

The scaffold acts as an intermediary structure that supports the low viscosity gel material during and after printing. It provides the mechanical framework necessary to maintain structural integrity while allowing the gel to be deposited with high precision containing viable cells.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Reliability

If low viscosity materials are printed, then cell viability is maintained, but shape preservation and structural protection deteriorate

Engineering Contradiction:
Improvecell viabilityVSAvoidshape preservation
Core Design Contradiction:
ReliabilityVSStability of the object's composition

Solution Approach 1:

The patent creates a composite structure where viable cells are encapsulated in low viscosity gel, which is then embedded within a high strength scaffold. This composite arrangement maintains cell viability while the scaffold provides shape preservation and structural protection.

Inventive Principle:
Principle #40Composite materials

Solution Approach 2:

The scaffold is prepared and positioned before the gel material is printed. This preliminary structural framework is established to guide and maintain the shape of the subsequent gel deposition, ensuring shape preservation while the gel contains viable cells.

Inventive Principle:
Principle #10Preliminary action

3Measurement precision

If conventional ink-jet printing is used, then cell placement accuracy is achieved, but structural complexity and porosity for nutrient transport are limited

Engineering Contradiction:
Improvecell placement accuracyVSAvoidstructural complexity
Core Design Contradiction:
Measurement precisionVSAdaptability or versatility

Solution Approach 1:

The patent employs composite materials that enable both precise cell placement and complex porous structures. The scaffold provides the complex porous architecture for nutrient transport while the gel with embedded cells achieves accurate cell placement, combining both functionalities in a single tissue construct.

Inventive Principle:
Principle #40Composite materials

Solution Approach 2:

The patent utilizes porous scaffold materials that facilitate nutrient and oxygen transport while maintaining structural integrity. The porous structure is integrated with the cell-containing gel to create a tissue construct that supports both accurate cell placement and effective nutrient diffusion.

Inventive Principle:
Principle #31Porous materials

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The system enables the production of stable, high-strength tissue constructs with precise cell placement and structural support, overcoming the limitations of low viscosity materials and maintaining the integrity of the printed structure post-implantation.

Implementation Method 1

a temperature regulator operatively associated with at least one (or both, or all) of the syringes for independently regulating the temperature of at least one (or both) of the pair of syringes

Methodology Applied
Scientific EffectThermal heating: Heating

Implementation Method 2

a temperature regulator operatively associated with at least one (or both, or all) of the syringes for independently regulating the temperature of at least one (or both) of the pair of syringes

Methodology Applied
Scientific EffectThermal cooling: Cooling

Implementation Method 3

a support stage for supporting an organ to be printed thereon

Methodology Applied
Scientific EffectMechanical support: Mechanical Force

Implementation Method 4

a positioning unit (as a single component or separate components) above, below, or adjacent the support stage and operatively associated therewith

Methodology Applied
Scientific EffectMechanical positioning: Mechanical Force

Data Source

PatentUS11801327B2Integrated organ and tissue printing methods, system and apparatus
Publication Date: 2023.10.31 WAKE FOREST UNIVERSITY HEALTH SCIENCES INC
  • US11801327B2 patent drawing
  • US11801327B2 patent drawing
  • US11801327B2 patent drawing

AI summary

A method of making an organ or tissue comprises: (a) providing a first dispenser containing a structural support polymer and a second dispenser containing a live cell-containing composition; (b) depositing a layer on said support from said first and second dispenser, said layer comprising a structural support polymer and said cell-containing composition; and then (c) iteratively repeating said depositing step a plurality of times to form a plurality of layers one on another, with separate and discrete regions in each of said layers comprising one or the other of said support polymer or said cell-containing composition, to thereby produce provide a composite three dimensional structure containing both structural support regions and cell-containing regions. Apparatus for carrying out the method and composite products produced by the method are also described.