OsDGD2β Gene Mutation for Stable Male Sterility in Rice Breeding
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Solution Overview
Problem
Current rice breeding methods lack effective genetic tools for inducing male sterility in rice, which is essential for hybrid seed production and heterosis utilization, with limited understanding and application of the OsDGD2β gene in this context.
Innovation Solution
The OsDGD2β gene is mutated using CRISPR/Cas9 gene editing to create a male sterile rice mutant, which affects anther and pollen development without impacting female fertility, enabling stable male sterility for hybrid breeding.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If traditional rice breeding methods are used, then breeding process is simple, but effective genetic tools for inducing male sterility are lacking
Solution Approach 1:
The patent applies CRISPR/Cas9 gene editing technology to mutate the OsDGD2β gene, changing the genetic parameters of rice to induce male sterility. This molecular breeding approach provides effective genetic tools while maintaining breeding efficiency, resolving the contradiction between traditional simple breeding methods and the need for reliable male sterility induction.
2Reliability
If OsDGD2β gene is mutated to induce male sterility, then male sterility is achieved, but understanding and application of the gene is limited
Solution Approach 1:
The patent systematically characterizes the OsDGD2β gene function through multiple experimental approaches including expression analysis, mutant phenotype observation, and complementation tests. The gene product itself serves as the subject of study, revealing its role in anther and pollen development. This comprehensive characterization fills the knowledge gap about the gene's function while maintaining reliable male sterility induction.
3Reliability
If male sterile rice mutants are created, then hybrid seed production is enabled, but female fertility may be affected
Solution Approach 1:
The patent demonstrates that mutation of the OsDGD2β gene specifically affects male reproductive structures (anthers and pollen) while leaving female reproductive structures (pistils) unaffected. This tissue-specific effect achieves stable male sterility while preserving female fertility, enabling the mutant to serve as a suitable female parent in hybrid seed production.
Data Source
AI summary
A new use of OsDGD2β gene in breeding of male sterile rice materials is disclosed. The OsDGD2β gene deletion mutant created by technologies such as CRISPR/Cas9 gene editing is stable in male sterility, with no negative impact on female fertility and other traits (such as photosynthesis), making it uniquely superior in cross breeding and utilization of heterosis.