OsRNR10 Gene Root Architecture Nitrogen Efficiency
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for improving rice yield are inefficient due to excessive nitrogen fertilizer use, which does not significantly increase yield but decreases economic and ecological benefits, and the genetic mechanisms of nitrogen and auxin interaction in rice root system development are not well understood.
Innovation Solution
The use of the OsRNR10 gene in rice, specifically its promoter sequence, to optimize root system development and nitrogen use efficiency, where a 3,496-bp deletion in the japonica rice promoter region leads to higher expression and sensitivity to nitrogen, while its absence in indica rice results in better root architecture and nitrogen uptake.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If nitrogen fertilizer input is increased to improve crop yield, then crop yield increases slightly, but economic and ecological benefits decline significantly
Solution Approach 1:
The invention changes the genetic parameter of rice by introducing the OsNLP4 gene, which alters the plant's response to nitrogen. This enables the rice to achieve optimal growth and yield with reduced nitrogen fertilizer input, thereby improving nitrogen use efficiency and reducing the loss of economic and ecological benefits associated with excessive fertilizer application
2Shape
If external nitrogen source level is increased to enhance root system development, then root system architecture improves, but nitrogen use efficiency decreases
Solution Approach 1:
The OsNLP4 gene enables the rice plant to self-regulate its nitrogen uptake and utilization. The gene product acts as a transcription factor that controls the expression of nitrogen transporters and metabolic enzymes, allowing the plant to efficiently acquire and utilize nitrogen from the soil without requiring excessive external nitrogen sources, thus maintaining both healthy root development and high nitrogen use efficiency
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The OsRNR10 gene promotes stability through mono-ubiquitination of OsDNR1, inhibiting auxin accumulation and root growth, enhancing nitrogen use efficiency and yield by optimizing root system architecture, and the 3,496-bp deletion serves as a marker for determining rice root system development and nitrogen use efficiency.
Implementation Method 1
OsRNR10 promotes its stability through a mono-ubiquitination modification of a K53 site of an auxin synthesis inhibiting factor OsDNR1
Data Source
AI summary
Use of an indica rice OsRNR10 gene in enhancing rice root system development, and improving nitrogen use efficiency and yield, where sequences of the indica rice OsRNR10 gene are shown in SEQ ID NOs: 1 and 2. 82 IRAT261-carrying single segment substitution lines under the background of Huajingxian 74 are utilized to carry out a quantitative trait locus (QTL) analysis. A nitrogen-response root system development regulatory factor OsRNR10 is further finely mapped. The indica rice haplotype OsRNR10 reduces the expression level, and enables indica rice to have a better root system architecture and a higher nitrate nitrogen uptake rate. By introducing an excellent allelic variation of OsRNR10 in the indica rice into japonica rice, the root system development of the japonica rice can be enhanced, and the nitrogen use efficiency and yield of the japonica rice can be improved.