OsSHM4 Gene Mutants Enhance Selenium Concentration in Rice
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Solution Overview
Problem
Rice often has low selenium content, which is insufficient to meet dietary needs in selenium-poor regions, and existing methods do not effectively enhance selenium concentrations in rice shoots without affecting agronomic traits.
Innovation Solution
Development of rice serine hydroxymethyltransferase gene OsSHM4 mutants that increase the absorbing capacity of rice roots to sulfate and selenate, thereby enhancing sulfur and selenium concentrations in rice shoots without influencing agronomic traits.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If selenium fertilizers are applied to increase selenium content in rice, then selenium concentration in rice is improved, but the absorption efficiency and distribution of selenium in different rice parts are not optimized
Solution Approach 1:
The patent applies parameter changes by modifying the OsSHM4 gene to alter the metabolic pathway parameters. The mutation changes the enzyme's substrate affinity or catalytic efficiency, thereby changing how selenium is processed and distributed in the plant, leading to improved absorption efficiency and higher selenium concentration in edible parts
Solution Approach 2:
The patent uses the OsSHM4 gene mutation as an intermediary mechanism to mediate selenium absorption and distribution. The mutated gene acts as a biological mediator that enhances the plant's ability to take up selenium from the soil and transport it to the shoots and grains, improving overall absorption efficiency without direct application of enhanced fertilizers
2Quantity of substance
If selenium content in rice is increased through fertilization, then dietary selenium needs are met, but agronomic traits and biomass may be affected
Solution Approach 1:
The patent changes the genetic parameter of the OsSHM4 gene to achieve higher selenium content while maintaining stable agronomic traits. The specific mutation (T461C) alters the enzyme's function in a way that selectively enhances selenium metabolism without disrupting other physiological processes that determine agronomic performance
Solution Approach 2:
The patent segments the effect of the gene mutation to specifically target selenium metabolism pathways while leaving other agronomic trait pathways unaffected. The OsSHM4 gene mutation acts independently on selenium-related metabolic processes, allowing selenium content to be improved without compromising overall plant health and agronomic characteristics
Data Source
AI summary
The present invention discloses rice serine hydroxymethyltransferase coded gene OsSHM4 mutants and application thereof. The rice serine hydroxymethyltransferase coded gene mutants are obtained in a manner that T at a 461st position of a CDS sequence region of a wild type rice OsSHM4 gene is mutated to C, so that coded amino acids thereof are mutated from leucine to proline. A CDS sequence of the wild type rice OsSHM4 gene is shown in SEQ ID NO. 1. After mutation of serine hydroxymethyltransferase coded genes OsSHM4, under a field experiment condition, S and Se content of rice shoots is remarkably improved without influencing biomass of rice. After wild type serine hydroxymethyltransferase coded genes OsSHM4 are genetically modified to complement the mutants, S and Se content of shoots of complemented strains is restored to that of wild type rice water planting.