OTU300 Archaeal Marker for Rapid Total Nitrogen Detection in Urban Green Soil
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Solution Overview
Problem
Traditional methods for detecting soil quality indicators in urban green land, such as total nitrogen content, are labor-intensive, time-consuming, and costly, making rapid detection of large quantities of samples difficult.
Innovation Solution
A primer-probe set comprising specific primers and a Taqman probe, along with a DNA molecule, is used for real-time fluorescent quantitative PCR to detect the abundance of archaea molecular marker OTU300, allowing for rapid and automatic detection of total nitrogen content in soil samples.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional physical and chemical detection methods are used to detect soil quality indicators, then measurement accuracy is maintained, but detection time increases and productivity decreases
Solution Approach 1:
The patent replaces traditional mechanical physical and chemical detection methods with a biological detection system using archaea molecular markers. By establishing a correlation between archaea abundance (detected via molecular biology techniques) and soil total nitrogen content, the invention substitutes complex chemical analysis with a more rapid biological indicator system that maintains measurement accuracy while significantly improving detection speed.
Solution Approach 2:
The invention changes the detection parameter from direct chemical measurement of soil nitrogen to indirect measurement through archaea molecular marker abundance. By using real-time fluorescent quantitative PCR to detect archaea 16S rRNA gene copies and correlating this with total nitrogen content, the system transforms a slow chemical analysis into a faster molecular biology-based detection while preserving measurement reliability.
2Reliability
If traditional detection methods are used, then comprehensive soil analysis is achieved, but labor cost and operational complexity increase
Solution Approach 1:
The patent extracts and isolates the key indicator for soil nitrogen assessment by focusing specifically on archaea molecular marker OTU300 abundance. Instead of performing comprehensive chemical analysis of all soil parameters, the invention extracts the critical relationship between archaea abundance and total nitrogen, simplifying the detection process while maintaining reliable assessment capability for this important soil quality indicator.
3Quantity of substance
If traditional methods are applied to large quantities of samples, then thorough analysis is possible, but time consumption and resource requirements increase significantly
Solution Approach 1:
The patent replaces time-consuming traditional chemical analysis with rapid molecular biology detection methods. By using real-time fluorescent quantitative PCR to detect archaea 16S rRNA gene copies, the system can process multiple samples in parallel with significantly reduced time requirements, enabling high-throughput detection of large numbers of soil samples while maintaining analytical reliability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid, cost-effective, and automated detection of total nitrogen content in soil, reducing sample and labor requirements, and facilitating comparisons across different plots.
Implementation Method 1
The labels can provide a signal that can be detected by fluorescence, radioactivity, colorimetry, gravimetry, X-ray diffraction or absorption, magnetism, enzyme activity, and so on. The labels can be a charged moiety (positive or negative) or optionally, can be neutral. The labels may include a nucleic acid or protein sequence or a combination thereof, as long as the sequence containing labels is detectable.
Implementation Method 2
The Taqman probe has a fluorescent group at its 5′end and a fluorescence quenching group at its 3′end. The fluorescent group may specifically be FAM. The fluorescence quenching group may specifically be TAMRA.
Data Source
AI summary
The present invention discloses a method for rapidly detecting the total nitrogen content of the soil in an urban green land by using archaea molecular marker OTU300. The present invention provides a DNA molecule (probe), as shown in SEQ ID NO.1 of the sequence listing. The present invention also protects the application of the probe in detecting or assisting in detecting the total nitrogen content of the soil. The present invention also protects the application of the probe in comparing the total nitrogen content of the soil in different plots. Using the method provided by the present invention to detect the total nitrogen content of the soil or compare the total nitrogen content of the soil of different plots has the following advantages: small sample demand, no need for pre-treatment, short required time, low labor cost, and realizing the rapid automatic detection of large quantities of samples. The present invention deserves to apply and promote in the evaluation of soil samples.