Oxalate Test Device Enzyme Detection
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Solution Overview
Problem
Current methods for monitoring oxalate levels in patients prone to kidney stone formation require 24-hour urine collections and are typically performed in diagnostic laboratories, making it difficult for patients to adhere to low oxalate diets and requiring sophisticated equipment.
Innovation Solution
A test device and method for detecting oxalate levels in biological samples, such as urine, using oxalate oxidase or oxalate decarboxylase enzymes in combination with indicator systems like MBTH, DMAB, PMS, and INT, which provide a colorimetric reaction for rapid and accurate detection at home or in clinical settings.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If 24-hour urine collection methods are used in diagnostic laboratories, then measurement precision of oxalate levels is improved, but device complexity and ease of operation deteriorate
Solution Approach 1:
The patent extracts the core detection function from complex laboratory equipment and implements it in a simplified test device format. The enzyme-based test strip or solution contains only the essential components (oxalate oxidase enzyme, chromogen, buffer) needed for oxalate detection, eliminating the need for sophisticated laboratory instrumentation while maintaining detection capability.
Solution Approach 2:
The test device performs self-contained detection without requiring complex laboratory equipment. The enzyme reaction occurs automatically when the test device contacts the urine sample, and the color change provides immediate visual results. This self-service mechanism eliminates the need for laboratory technicians and sophisticated equipment while enabling patients to perform monitoring at home.
2Measurement precision
If 24-hour urine collection methods are used in diagnostic laboratories, then measurement precision of oxalate levels is improved, but ease of operation deteriorates
Solution Approach 1:
The patent extracts the complex laboratory procedure and reduces it to a simple home-test format. Patients can collect a small urine sample and apply it to the test device without needing to maintain complex 24-hour collections or operate sophisticated equipment. The simplified procedure significantly improves ease of operation and patient compliance.
Solution Approach 2:
The test device enables patients to perform their own monitoring without requiring laboratory facilities or technician assistance. The self-contained design with visual color-change results allows patients to independently assess their oxalate levels and adjust their diets accordingly, greatly enhancing ease of operation and long-term compliance.
3Productivity
If rapid detection methods are implemented, then productivity is improved, but measurement precision may deteriorate
Solution Approach 1:
The test device incorporates pre-optimized enzyme concentrations and buffer compositions that ensure the reaction reaches completion within a specific time frame. The chromogen and enzyme are formulated in advance to provide sufficient reaction time for accurate measurement while enabling rapid results within minutes, balancing productivity and measurement precision.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables patients to monitor oxalate levels easily and quickly, enhancing compliance with dietary restrictions and providing immediate feedback on the impact of diet on oxalate levels without the need for sophisticated laboratory equipment.
Implementation Method 1
oxalate oxidase enzyme that catalyzes the oxidation of oxalate to form hydrogen peroxide
Implementation Method 2
oxalate oxidase
Implementation Method 3
The hydrogen peroxide reacts with 3-methyl-2-benzothiazolinone hydrazone and 3-(dimethylamino) benzoic acid in the presence of peroxidase to yield an indamine dye
Implementation Method 4
oxalate decarboxylase that catalyzes the decarboxylation of oxalate to formate and carbon dioxide
Implementation Method 5
formate dehydrogenase that converts the formate to carbon dioxide
Data Source
AI summary
The present invention features compositions and methods for the detection or measurement of oxalate in a sample. Such compositions include test devices that provide for the rapid and accurate detection of oxalate in a sample from a biological fluid. Advantageously, the compositions can be used to monitor the oxalate levels of a patient at a point of care (e.g., at the patient's home, clinic, physician's office, or other clinical setting).