Oxidase Biosensor Dissolved Oxygen Correction
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Solution Overview
Problem
Existing biosensors using oxidation-reduction enzymes face challenges in accurately measuring analytes in blood samples due to the influence of dissolved oxygen, leading to potential over-administration of insulin and undetected hypoglycemia, and require costly investments and higher enzyme costs when using alternative methods.
Innovation Solution
A method involving an oxidase-based biosensor with multiple independent electrode systems and oxidase-containing reagents, which react with the target object under different conditions to obtain measurement values, allowing for correction of dissolved oxygen influences using a pre-set correction method without additional enzymes or electrodes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If a biosensor using GOD (glucose oxidase) is used to measure glucose in blood, then the measurement can be performed with lower enzyme costs, but the measurement accuracy deteriorates due to the influence of dissolved oxygen
Solution Approach 1:
The invention divides the measurement system into two separate electrode systems: a first electrode system that measures both glucose and dissolved oxygen (using GOD), and a second electrode system that measures only dissolved oxygen (using a non-specific electrode). By segmenting the measurement functions, the system can separately quantify and subtract the dissolved oxygen interference from the glucose measurement, thereby maintaining measurement accuracy while using cost-effective GOD enzyme.
Solution Approach 2:
The invention introduces dissolved oxygen as an intermediary variable that mediates the interference effect. By measuring dissolved oxygen concentration through the first electrode system and using it to correct the glucose measurement, the system transforms the harmful interference into a correctable parameter, enabling accurate glucose measurement despite the presence of dissolved oxygen.
2Measurement precision
If a third electrode is added to correct dissolved oxygen influence, then measurement accuracy improves, but capital investment and device complexity increase
Solution Approach 1:
The invention makes the first electrode system multi-functional by enabling it to serve both as a glucose sensor (through GOD-mediated reaction) and as a dissolved oxygen sensor (through direct oxygen reduction). This universality eliminates the need for a separate third electrode dedicated to oxygen measurement, reducing device complexity and capital investment while maintaining the ability to correct for dissolved oxygen interference.
Solution Approach 2:
The invention merges the glucose sensing function and dissolved oxygen sensing function into a single first electrode system. By combining these functions, the system avoids the need for additional electrodes and simplifies the overall device structure, thereby reducing capital investment and device complexity while achieving accurate glucose measurement through dissolved oxygen correction.
3Measurement precision
If GDH (glucose dehydrogenase) is used instead of GOD to avoid dissolved oxygen influence, then measurement accuracy improves, but enzyme costs increase
Solution Approach 1:
The invention segments the measurement functions between two electrode systems using GOD: the first electrode system measures both glucose and dissolved oxygen, while the second electrode system measures only dissolved oxygen. This segmentation allows the use of cost-effective GOD enzyme in both systems, avoiding the need for expensive GDH, while still achieving accurate glucose measurement by subtracting the dissolved oxygen contribution calculated from the second electrode's measurement.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate determination of analyte concentrations in the presence of dissolved oxygen, reducing enzyme costs and capital investment while correcting oxygen influences without additional enzymes or electrodes, ensuring reliable glucose measurement.
Implementation Method 1
An enzyme sensor performs a quantitative analysis of an analyte by reducing an electron acceptor (mediator) with electrons generated by reaction between an enzyme and a target object to be measured (substrate) contained in a sample solution
Implementation Method 2
electrochemically measuring an oxidation-reduction degree of the electron acceptor
Data Source
AI summary
A method for measuring a target object in a sample by using an oxidase, wherein the influence of dissolved oxygen in the sample can be corrected, is provided. The method comprises: obtaining measurement values by causing the target object in the sample to react with the oxidase under different conditions of two or more types; and performing a correction based on the obtained two or more measurement values and a correction method preliminarily set so as to correct the influence of dissolved oxygen in the sample.