Oxidative Chromogenic Compound for Stable Colorimetric Analysis
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Solution Overview
Problem
Current colorimetric methods for measuring glucose and cholesterol in blood suffer from time-dependent reagent degradation and limited sensitivity, making them less accurate and slower for high-precision measurements, especially in detecting smaller component concentrations.
Innovation Solution
Development of an oxidative chromogenic compound represented by a specific chemical formula, supported on a membrane carrier, which enhances solubility, stability, and sensitivity when combined with a Trinder's reagent and enzymes, allowing for quick and accurate color reactions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional coloring reagents are used in colorimetric methods, then the measurement can be performed, but the reagent degrades over time leading to reduced accuracy and limited sensitivity
Solution Approach 1:
The patent modifies the molecular structure of conventional coloring reagents by introducing specific substituent groups (such as sulfonic acid groups, aromatic hydrocarbon groups) to change the chemical parameters of the reagent. This structural modification enhances the reagent's stability against degradation while maintaining its colorimetric properties, thereby resolving the contradiction between reagent stability and measurement accuracy
Solution Approach 2:
The invention creates composite chromogenic compounds that combine multiple functional groups and structural elements within a single molecular framework. These composite structures integrate the stability-providing groups (like sulfonic acid groups) with the colorimetrically active groups, achieving both long-term stability and high measurement precision simultaneously
2Measurement precision
If conventional coloring reagents are used, then the measurement can be performed, but the sensitivity is limited especially for detecting smaller component concentrations
Solution Approach 1:
The patent changes the optical and chemical parameters of the coloring reagent through molecular structure modification. The introduction of specific aromatic groups and conjugated systems enhances the molar absorptivity and color intensity of the reagent, thereby improving detection sensitivity for low concentrations of analytes while expanding the detectable concentration range
3Ease of operation
If the reagent concentration varies, then the measurement can be performed, but measurement errors increase due to concentration variations
Solution Approach 1:
Instead of trying to maintain perfectly constant reagent concentration through precise preparation and storage conditions, the patent inverts the approach by designing a reagent whose measurement performance is insensitive to concentration variations. The modified molecular structure provides a linear and stable color response over a broader concentration range, reducing measurement errors even when concentration varies slightly
Solution Approach 2:
The patent changes the response characteristics of the reagent by modifying its molecular structure to exhibit a more linear and stable colorimetric response over a wider concentration range. This parameter change makes the measurement process more robust against concentration variations, reducing operational complexity and measurement errors
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The oxidative chromogenic compound improves measurement sensitivity and accuracy by maintaining stability and solubility, reducing measurement errors due to reagent concentration variations and enabling faster results with improved solubility and uniform reactions.
Implementation Method 1
oxidizing a coloring reagent with the active oxygen to generate a pigment
Implementation Method 2
changing the hydrogen peroxide into active oxygen by peroxidase
Implementation Method 3
having a specific oxidase (oxidative enzyme) acted on a substance to be measured to generate hydrogen peroxide
Data Source
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AI summary
It is an object of the present invention to provide a means for measuring a concentration of various components rapidly with high accuracy and high sensitivity. The present invention is to provide an oxidative chromogenic compound or salt thereof represented by the following chemical formula (1): wherein R1 and R2 are each independently a linear or branched alkyl group having 1 to 5 carbon atoms; R3, R4, R5, R6 and R7 are each independently a hydrogen atom, a carboxyl group or a sulfonic acid group, or salt thereof, provided that at least one of R3 to R7 is a carboxyl group or a sulfonic acid group, or salt thereof, and the others are hydrogen atoms, and the production method thereof, and the reagent composition and test instrument using the same.