Oxidative Stress Measurement via ROS Induction and Probiotic Control
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Solution Overview
Problem
Current methods fail to effectively measure and control oxidative stress in humans and animals, particularly due to the induction of reactive oxygen species (ROS) by elevated inorganic sulfur consumption, which is linked to ultra-exogenous sulfide formation (USF) caused by sulfate-reducing bacteria (SRB) in the gut.
Innovation Solution
A method involving the collection and processing of blood samples to induce ROS production, using specific agents to measure ROS levels, and comparing these levels to determine oxidative stress, alongside dietary management and probiotic use to regulate inorganic sulfur consumption and SRB abundance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If elevated levels of inorganic sulfur are consumed, then sulfate reducing bacteria abundance increases and ultra-exogenous sulfide formation occurs, but oxidative stress increases due to ROS production in blood cells
Solution Approach 1:
The patent introduces methanogenic probiotics that convert the harmful effect of inorganic sulfur consumption into beneficial methane production. These probiotics compete with sulfate reducing bacteria for inorganic sulfur, transforming the harmful sulfide formation pathway into a beneficial methane generation process that reduces oxidative stress while maintaining sulfur utilization.
Solution Approach 2:
The patent uses methanogenic probiotics as intermediary organisms that mediate between inorganic sulfur consumption and oxidative stress. These probiotics act as a buffer by consuming inorganic sulfur through methanogenesis, preventing direct conversion to harmful sulfides and ROS, thus reducing oxidative stress without blocking sulfur intake.
2Quantity of substance
If sulfate reducing bacteria abundance is high, then inorganic sulfur consumption increases, but reactive oxygen species production increases causing oxidative stress
Solution Approach 1:
The patent converts the harmful role of inorganic sulfur consumption by sulfate reducing bacteria into a beneficial process through methanogenic probiotics. These probiotics utilize the same inorganic sulfur substrate but direct it toward methane production rather than sulfide formation, eliminating ROS generation while maintaining sulfur metabolism.
Solution Approach 2:
The patent employs methanogenic probiotics as functional copies or alternatives to sulfate reducing bacteria. Both groups consume inorganic sulfur, but the probiotic copy follows a different metabolic pathway (methanogenesis vs. sulfide production), resulting in beneficial rather than harmful outcomes.
3Measurement precision
If reactive oxygen species are induced in blood cells, then oxidative stress measurement becomes possible, but harmful effects of oxidative stress increase
Solution Approach 1:
The patent performs preliminary measurement of oxidative stress by inducing ROS in controlled blood samples before implementing therapeutic interventions. This allows baseline assessment of oxidative stress status, enabling monitoring of treatment effectiveness and guiding personalized dietary or probiotic interventions to reduce harmful effects.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for the accurate measurement and control of oxidative stress, reducing its harmful effects and associated diseases by managing inorganic sulfur intake and using methanogenic probiotics to decrease SRB abundance, thereby reducing ROS-positive blood cells.
Implementation Method 1
adding a first agent to the second sample, the first agent being configured to induce reactive oxygen species (ROS) in blood cells of the second sample
Implementation Method 2
adding a second agent to each of the first and second samples following the isolating step, wherein adding the second agent at least partially reacts with ROS present in the plasma of each of the first and second samples
Data Source
AI summary
The present application describes methods and systems for measuring and controlling oxidative stress in animals and humans. The degree of oxidative stress can be measured directly by inducing all of the blood cells to produce excessive reactive oxygen species (ROS) by exposure to an elevated concentration sulfide or other ROS inducing chemical and measuring the fluorescence intensity of a fluorescent dye or color intensity of dye that reacts with ROS. Oxidative stress can be reduced by reducing dietary sulfur, consumption of a methanogenic probiotic, or apheresis methods to replace ROS-positive blood cells with normal blood cells. Plasma oxidative stress can be compared in venous and arterial blood samples to evaluate small vessel disease. Oxidative stress can be increased by increasing dietary sulfur or the use of an intravenous method that exposes blood cells to an elevated blood concentration of sulfide or other ROS inducing chemical.